Merck, A1720-5G, G 418 disulfate salt

Name: G 418 disulfate salt
Brand: Merck
SKU: A1720-5G

Size:5 g, Antibiotic G418, powder, BioReagent, suitable for cell culture

Available Catalog Numbers

A1720-1G A1720-25G A1720-5G

Catalog Number: A1720-5G

Pricing

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Category

Antibiotics & Antimycotics

Pack Size5 g

TagsCell Culture & Analysis Cell Culture Supplements & Reagents Antibiotics

CollectionsMerck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

General description
Chemical structure: aminoglycoside
G-418 is an aminoglycoside antibiotic, which is produced by Micromonospora rhodorangea NRRL 5326 strain. It has a broad range of antibacterial activity both in vivo and in vitro. It also shows activity against protozoa, cecal amoebiasis, and helminthes. It is commonly known as geneticin selective antibiotic.
Application
G418 disulfate salt is an aminoglycoside antibiotic similar in structure to gentamicin used for selection and maintenance of prokaryotic and eukaryotic cells transfected with a iNOS promoter construct and neomycin resistance gene†. Optimal concentrations for selection and maintenance vary by cell lines. Bacteria and algae require 5 μg/mL or less while animal cells may require 300-500 μg/mL.
Biochem/physiol Actions
G-418 inhibits protein synthesis at the early stages of elongation, post-initiation, at the initiation stage of translation as well as just before the initiation stage of translation.
Preparation Note
G418 powder is soluble in water at 100 mg/mL.
Other Notes
Keep container tightly closed in a dry and well-ventilated place. Keep in a dry place.
Disclaimer
The product is stable for two years when stored at 2-8°C. Stock solutions are stable at 37°C for 8 days.

Specifications

General

UNSPSC Code	12352207
PubChem Substance ID	24890581
NACRES	NA.76
Web Name Suffix Override	powder, non-animal origin, suitable for cell culture, BioReagent
packaging	pkg of 5 g
SMILES string	OS(O)(=O)=O.OS(O)(=O)=O.CN[C@H]1[C@H](O)[C@@H](OC[C@@]1(C)O)O[C@H]2[C@H](N)C[C@H](N)[C@@H](O[C@H]3O[C@H]([C@H](C)O)[C@@H](O)[C@H](O)[C@H]3N)[C@@H]2O
InChI	1S/C20H40N4O10.2H2O4S/c1-6(25)14-11(27)10(26)9(23)18(32-14)33-15-7(21)4-8(22)16(12(15)28)34-19-13(29)17(24-3)20(2,30)5-31-19;21-5(2,3)4/h6-19,24-30H,4-5,21-23H2,1-3H3;2(H2,1,2,3,4)/t6?,7-,8+,9+,10+,11-,12-,13-,14+,15+,16-,17-,18+,19-,20+;;/m0../s1
InChI key	UHEPSJJJMTWUCP-NKCAIAFTSA-N
product line	BioReagent
form	powder
optical activity	[α]/D (Specific Rotation: +104.4o (c=0.3% in H2O at 26oC))
potency	≥720 μg per mg (Dried basis)
mol wt	692.7
technique(s)	cell culture \| mammalian: suitable
color	white to off-white
solubility	H2O: 100 mg/mL (As a stock solution. Stock solutions should be stored at 2-8°C. Stable at 37°C for 8 days.)

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