Merck, A3059-100G, Bovine Serum Albumin

Name: Bovine Serum Albumin
Brand: Merck
SKU: A3059-100G

Size:100 g, Albumin bovine serum, BSA, Bovine albumin, heat shock fraction, protease free, essentially globulin free, pH 7, ≥98%

Available Catalog Numbers

A3059-100G A3059-500G A3059-50G

Catalog Number: A3059-100G

Pricing

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Category

Protein-Free Media

Pack Size100 g

TagsCell Culture & Analysis Cell Culture Supplements & Reagents Albumins & Transport Proteins

CollectionsMerck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

General description
Bovine serum albumin (BSA) has an important role in the regulation of blood colloidal osmotic pressure. BSA is the major protein in bovine serum, and is composed of 583 amino acids with a calculated molecular weight of 66,430 Daltons. BSA is used in many different applications and fields, such as:

Protein quantitation (as a standard)
Immunochemistry (as a blocking agent)
Cell culture (as a nutrient)

This particular BSA is specifically evaluated and tested to have very low protease content / for protease-free status. Protease-free BSA may be used in applications like:

Enzyme assays
Protein-based assays
Protease-sensitive techniques that include:

a) Enzyme immunoassay (EIA)
b) Nucleic acid hybridization
c) Radioimmunoassay (RIA)
Application
Bovine Serum Albumin has been used in luminescence assays.
Biochem/physiol Actions
Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.
Features and Benefits

Heat shock fractionated
Protease-free
Essentially globulin-free

Preparation Note
Prepared using heat shock fractionation
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.
Other Notes
2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Specifications

General

UNSPSC Code	12352202
EC Number	232-936-2
NACRES	NA.27
MDL number	MFCD00130384
eCl@ss	32160409
biological source	bovine
assay	≥98%
form	lyophilized powder
Gene Information	bovine ... ALB(280717)
Quality Level	300
purified by	heat shock fractionation
packaging	poly bottle of
origin	USA origin
technique(s)	ELISA: suitable
impurities	essentially globulin free
loss	≤5%
pH	7
solubility	water: soluble (40 mg/ml)
UniProt accession no.	P02769
foreign activity	BT Virus, none detected
storage temp.	2-8°C

Ordering

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