Merck, C0775-100G, Crystal Violet

Name: Crystal Violet
Brand: Merck
SKU: C0775-100G

Size:100 g, Basic Violet 3, Gentian Violet, Hexamethylpararosaniline chloride, Methyl Violet 10B, certified by the BSC

Available Catalog Numbers

C0775-100G C0775-25G

Catalog Number: C0775-100G

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Category

Special Stains

Pack Size100 g

TagsClinical & Diagnostics Tissue Diagnostics Dry Dyes & Certified Stains

CollectionsMerck Tissue Diagnostics – Universal Reagents for Histology & IHC Merck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

Application
Certified for use with the Gram stain for bacteria, Flemming triple stain with iodine for chromosomes, Lieb′s amyloid stain and Newton′s crystal violet-iodine technique for chromatin and nucleoli.
Crystal violet is used as an active component, primary stain, of Gram stain for differentiation of Gram-negative versus Gram-positive bacteria.
It has been used to check cell viability. It has also been used for the staining of cells to study cell migration and invasion.
Biochem/physiol Actions
Crystal violet can be used for DNA visualization in agarose gels. The dye is used only in the presence of high concentrations of DNA. Crystal violet is also used for the staining of bacteria in gram staining technique. It is also used for the staining of plant chromosomes. Crystal Violet also helps in colorimetric measurement of cell viability.

Specifications

General

UNSPSC Code	12171500
Web Name Suffix Override	Dye content, ≥90%, certified by the Biological Stain Commission, powder
NACRES	NA.47
PubChem Substance ID	57653980
EC Number	208-953-6
MDL number	MFCD00011750
Beilstein/REAXYS Number	3580948
Colour Index Number	42555
SMILES string	[Cl-].CN(C)c1ccc(cc1)\C(c2ccc(cc2)N(C)C)=C3/C=C\C(C=C3)=[N+](/C)C
InChI key	ZXJXZNDDNMQXFV-UHFFFAOYSA-M
InChI	1S/C25H30N3.ClH/c1-26(2)22-13-7-19(8-14-22)25(20-9-15-23(16-10-20)27(3)4)21-11-17-24(18-12-21)28(5)6;/h7-18H,1-6H3;1H/q+1;/p-1
agency	certified by the BSC
form	powder
composition	Dye content, ≥90%
technique(s)	microbe id \| staining: suitable
color	green to very dark green
pH	2.5 — 3.5 (20 °C)
mp	205 °C (dec.) (lit.)
solubility	H2O: 1 mg/mL
density	1.19 g/cm3 at 20 °C
antibiotic activity spectrum	fungi
application(s)	diagnostic assay manufacturinghematologyhistology

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