Merck, C9999-1000ML, Citrate Buffer, pH 6.0, 10×, Antigen Retriever

Name: Citrate Buffer, pH 6.0, 10×, Antigen Retriever
Brand: Merck
SKU: C9999-1000ML

Size:1000 mL, 1, 2, 3-Propanetricarboxylic acid, 2-hydroxy-, mixt. with sodium hydroxide (Na(OH)) and sodium 2-hydroxy-1, 2, 3-propanetricarboxylate, for immunohistochemistry

Available Catalog Numbers

C9999-1000ML C9999-100ML

Catalog Number: C9999-1000ML

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Category

Microbiological Stains

Pack Size1000 mL

TagsClinical & Diagnostics Tissue Diagnostics Hematology Stains

CollectionsMerck Tissue Diagnostics – Universal Reagents for Histology & IHC Merck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

Application
Citrate Buffer, pH 6.0, 10×, Antigen Retriever has been used as a heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies or DNA fluorescent in situ hybridization. In immunohistochemistry (IHC), most commonly used fixatives such as formalin mask tissue antigens (cellular, membrane, and nuclear) by their intrinsic crosslinking. This masking results in poor or no staining in IHC. The use of Citrate buffer, pH 6.0, or other antigen retrieval solutions on FFPE tissue sections improves accessibility of antibodies to tissue antigens.
Preparation Note
Dilute the Citrate buffer, pH 6.0, 10x, Antigen Retriever 10-fold with water to prepare a 1x Working Solution, e.g., dilute 10 mL of 10x concentrate with 90 mL of water.
Store the product at 2–8 °C. Do Not Freeze to prevent possible precipitation.
Other Notes
Occasionally the buffer may contain a yellowish tinge. This is due to preservative and will not affect the function of the buffer.
Store the prepared 1′ Citrate buffer, pH 6.0, Working Solution at 2–8 °C.

Specifications

General

NACRES	NA.47
UNSPSC Code	12352107
form	liquid
pH	6.0
application(s)	hematologyhistology
storage temp.	2-8°C
Quality Level	100

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