Merck, E2129-10G, Evans Blue

Name: Evans Blue
Brand: Merck
SKU: E2129-10G

Size:10 g, Direct Blue 53, Dye content ≥75 %

Available Catalog Numbers

E2129-100G E2129-10G E2129-50G

Catalog Number: E2129-10G

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Category

Special Stains

Pack Size10 g

TagsClinical & Diagnostics Tissue Diagnostics Hematology Stains

CollectionsMerck Tissue Diagnostics – Universal Reagents for Histology & IHC Merck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

General description
Evans blue (EB) C.I. 23860 is a synthetic bis-azo dye also known as T-1824 and Direct Blue 53. EB dye with higher water solubility has a strong affinity towards serum albumin, making it a high molecular weight protein tracer.
Application
Evans blue is a well-known vital stain with applications in histology and fluorescent microscopy. Evans blue has been used to check cell viability and study blood-brain barrier (BBB) permeability. Additional applications include the demonstration of specific pituitary cell types, selective staining of neutrophil plasma membranes, and fluorescence microscopy as a quenching agent to suppress unwanted autofluorescence.
Biochem/physiol Actions
Evans blue is a non-permeating dye. In presence of plasma membrane damage, the dye enters in the cytoplasm and nucleus, thereby staining them blue. Evans blue is used for checking cell viability. It is also used to study BBB (blood-brain barrier) permeability. The dye binds to albumin and hence indicates BBB breakdown to protein.

Specifications

General

Web Name Suffix Override	Dye content ≥75 %, Powder
NACRES	NA.47
PubChem Substance ID	24894456
UNSPSC Code	12171500
EC Number	206-242-5
MDL number	MFCD00004021
Colour Index Number	23860
InChI key	ATNOAWAQFYGAOY-GPTZEZBUSA-J
InChI	1S/C34H28N6O14S4.4Na/c1-15-11-17(3-7-21(15)37-39-23-9-5-19-25(55(43,44)45)13-27(57(49,50)51)31(35)29(19)33(23)41)18-4-8-22(16(2)12-18)38-40-24-10-6-20-26(56(46,47)48)14-28(58(52,53)54)32(36)30(20)34(24)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4/b39-37+,40-38+;;;;
SMILES string	[Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1\N=N\c2ccc3c(cc(c(N)c3c2O)S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(\N=N\c5ccc6c(cc(c(N)c6c5O)S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4
form	powder
composition	Dye content, ≥75%
technique(s)	titration: suitable
application(s)	diagnostic assay manufacturinghematologyhistology
storage temp.	room temp
Gene Information	human ... PTPN1(5770), PTPRF(5792)
Quality Level	200

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