Merck, F2456-100G, Fluorescein (free acid)

Name: Fluorescein (free acid)
Brand: Merck
SKU: F2456-100G

Size:100 g, Dye content 95 %

Available Catalog Numbers

F2456-100G F2456-2.5G F2456-500G

Catalog Number: F2456-100G

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Category

Special Stains

Pack Size100 g

TagsClinical & Diagnostics Tissue Diagnostics Hematology Stains

CollectionsMerck Tissue Diagnostics – Universal Reagents for Histology & IHC Merck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

General description
Fluorescein (free acid, CI 45350) is a xanthene dye. Fluorescein (free acid) molecule can be divided into two parts. One part contains xanthene moiety which acts as fluorophore and the other part of the molecule bears benzene moiety which is the fluorescence-controlling moiety.
Application
Fluorescein (free acid) has been used as a staining agent in retinal imaging.
Fluorescein (free acid) is widely used as a fluorescent stain and trace for many living cells and tissue applications. It is also suitable as a marker for the study of motility of bile canaliculi. Some of the clinical applications are evaluating the ocular surface and delineation of brain regions lacking a blood-brain barrier to permit accurate surgical resection of malignant glioma. Fluorescein angiography helps investigate changes in retinal blood supply. Fluorescein also plays a role as a physiological tracer to characterize the salmon′s intestinal permeability (Schep et al., 1998), and for the fluorophotometric measurement of aqueous humor flow. It is widely employed as a quantum yield standard.

Specifications

General

NACRES	NA.47
PubChem Substance ID	24894822
UNSPSC Code	12171500
Colour Index Number	45350:1
Web Name Suffix Override	Dye content 95%, Powder
EC Number	219-031-8
MDL number	MFCD00005050
Beilstein/REAXYS Number	94324
InChI	1S/C20H12O5/c21-11-5-7-15-17(9-11)24-18-10-12(22)6-8-16(18)20(15)14-4-2-1-3-13(14)19(23)25-20/h1-10,21-22H
InChI key	GNBHRKFJIUUOQI-UHFFFAOYSA-N
SMILES string	Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5ccccc45)c1
form	powder
composition	Dye content, 95%
technique(s)	titration: suitable
color	orange to red-brown
mp	320 °C (lit.)
λ<sub>max</sub>	496 nm
ε (extinction coefficient)	≥12000 at 282-286 nm
application(s)	diagnostic assay manufacturinghematologyhistology
storage temp.	room temp
Quality Level	200

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