Merck, G1890-100G, Gelatin from porcine skin

Name: Gelatin from porcine skin
Brand: Merck
SKU: G1890-100G

Size:100 g, powder, gel strength ~300 g Bloom, Type A, BioReagent, suitable for electrophoresis, suitable for cell culture

Available Catalog Numbers

G1890-100G G1890-1KG G1890-500G

Catalog Number: G1890-100G

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Category

Differentiation Factors

Pack Size100 g

TagsCell Culture & Analysis Cell Culture Supplements & Reagents Attachment Factors

CollectionsMerck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

General description
Gelatin from porcine skin is generated from the acidic digestion of collagen and is referred as type A. Porcine gelatin comprises majorly glycine, proline and hydroxyproline. Gelatin takes up random coil structure after digestion from the triple helical collagen. It differs from type B bovine gelatin at the N-terminal sequence.
Application
Gelatin has been used in many applications. It has been used in coating cell culture to improve attachment of cells, being added to PCR to stabilize Taq DNA, as a blocking reagent in western blotting, ELISA, and immunochemistry, and as a component of media for species differentiation in bacteriology. As a biocompatible polymer, it has been used as a delivery vehicle for release of active biomolecules and in generation of scaffolds for tissue engineering applications. In the pharmaceutical industry, gelatin can be used as a suspending and encapsulating agent, among other applications. This product is recommended for use as a cell culture substratum at 1-5 μg/cm2 or 0.5-50 μg/mL. The optimal concentration does depend on cell type as well as the application and research objectives.
Features and Benefits

Sterile gelatin sourced from porcine skin.
Free from proteases.
In the form of gelatin powder for convenient and easy handling.
Versatile for use in cell culture and electrophoresis techniques.
Provides adequate surface coverage for various applications.

Preparation Note
This product is derived from porcine skin. Gelatin is soluble in hot than in cold water. It is practically insoluble in most organic solvents such as alcohol, chloroform, carbon disulfide, carbon tetrachloride, ether, benzene, acetone, and oils. The Bloom number, determined by the Bloom gelometer, is an indication of the strength of a gel formed from a solution of the known concentration. The Bloom number is proportional to the average molecular mass. Bloom numbers of porcine skin Gelatin vary from 90 to 300 g. This product has a gel strength of approximately 300.
Other Notes
Gelatin is a heterogeneous mixture of water-soluble proteins of high average molecular masses, present in collagen. Proteins are extracted by boiling the relevant skin, tendons, ligaments, bones, etc. in water. Type A gelatin is derived from acid-cured tissue. Type B is derived from lime-cured tissue.
Disclaimer
Dry gelatin, when stored in airtight containers at room temperature, will remain unchanged for many years. When heated at 100°C in the presence of air, it swells becomes soft and disintegrates to a carbonaceous mass with evolution of pyridine bases and ammonia.

Specifications

General

MDL number	MFCD00081638
UNSPSC Code	12352202
EC Number	232-554-6
Web Name Override	Porcine Gelatin
Web Name Suffix Override	from porcine skin, Type A, powder, gel strength ~300 g Bloom, suitable for electrophoresis and cell culture
NACRES	NA.75
biological source	Porcine skin
sterility	sterile
type	Type A
product line	BioReagent
form	powder
mol wt	50—100 kDa
packaging	pkg of 1 kg
concentration	70—90% Biuret
technique(s)	cell culture \| mammalian: suitable
surface coverage	100‑200 μg/cm2
solubility	H2O: soluble 50 mg/mL, clear to hazy, faintly yellow
web metadata keyword.default	cell attachment substrate, cell adherence substrate
shipped in	ambient
storage temp.	room temp
Quality Level	200

Ordering

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