Merck, M1255-10G, Calcein Blue

Name: Calcein Blue
Brand: Merck
SKU: M1255-10G

Size:10 g, 4-Methylumbelliferone-8-methyliminodiacetic acid, fluorescent dye

Available Catalog Numbers

M1255-10G M1255-1G

Catalog Number: M1255-10G

Pricing

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Category

Special Stains

Pack Size10 g

TagsClinical & Diagnostics Tissue Diagnostics Hematology Stains

CollectionsMerck Tissue Diagnostics – Universal Reagents for Histology & IHC Merck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

General description
Calcein Blue is a cell-permeable dye that stains viable cells. Upon entering the cell, intracellular esterases cleave the acetoxymethyl (AM) ester group, yielding the membrane-impermeable Calcein Blue fluorescent dye. The staining applications of Calcein blue include tumor tissues, mineralized tissues, bone, amino acids, and many more.
Application
Calcein blue has been used fluorescent labeling of the mineralised sections of bone. Calcein blue AM ester hydrolyzed to calcein blue is suitable as a a viability stain, both microscopically, and in fluorescence activated cell sorting systems. It is also used as a merker of living cells like Schwann cells at neuromuscular junctions.

Specifications

General

NACRES	NA.47
PubChem Substance ID	24896629
UNSPSC Code	12171500
Web Name Suffix Override	Powder
EC Number	259-129-8
MDL number	MFCD00006867
InChI key	RJPSHDMGSVVHFA-UHFFFAOYSA-N
InChI	1S/C15H15NO7/c1-8-4-14(22)23-15-9(8)2-3-11(17)10(15)5-16(6-12(18)19)7-13(20)21/h2-4,17H,5-7H2,1H3,(H,18,19)(H,20,21)
SMILES string	CC1=CC(=O)Oc2c(CN(CC(O)=O)CC(O)=O)c(O)ccc12
form	powder
technique(s)	titration: suitable
color	white to light yellow
solubility	1 M NaOH: 50 mg/mL
fluorescence	λex 322 nm; λem 445 nm in methanol
suitability	suitable for indicator for EDTA titration
application(s)	diagnostic assay manufacturinghematologyhistology
storage temp.	room temp
Quality Level	200

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