Merck, P7252-1G, Phospho(enol)pyruvic acid tri(cyclohexylammonium) salt

General description
Phospho(enol)pyruvic acid is a highly functionalized small molecule. It plays a major role in the metabolism.
Application
Phospho(enol)pyruvic acid tri(cyclohexylammonium) salt has been used:
in the pyruvate kinase/lactate dehydrogenase enzyme kinetic assay
in the reaction mixture to determine fructokinase activity of Zobellia galactanivorans
in luminometry to determine the amount of ADP formed by measuring the increase in light output

Biochem/physiol Actions
Phospho(enol)pyruvic acid is significant in high-phosphate group-transfer function. PEP plays a major role in PEP:sugar phosphotransferase system (PTS) mediated phosphorylation and transport of carbohydrates across the bacterial membrane. It functions to generate natural phosphonates catalyzed by PEP mutase. It plays a key role in the peptidoglycan cell wall biosynthesis.
Phospho(enol)pyruvic acid (PEP) is involved in glycolysis and gluconeogenesis. In glycolysis, PEP is metabolized by pyruvate kinase to yield pyruvate. In plants, PEP is involved in the formation of aromatic amino acids as well as in the carbon fixation pathway.