Merck, R5636-1ML, Ribonucleic acid, transfer from baker′s yeast (S. cerevisiae)

General description
Transfer RNA (tRNA) is isolated from baker′s yeast by phenol-chloroform extraction and ethanol precipitation. tRNAs are approximately 80 nucleotides long RNA molecules with a cloverleaf shaped secondary structure and a tertiary L-shaped structure.
Application
Suitable for use as a carrier in nucleic acid purification and precipitation.
Ribonucleic acid, transfer from baker′s yeast (S. cerevisiae) has been used:
in carrier solution as a part of control used for reverse transcriptase – quantitative polymerase reaction (RT-qPCR) assay
as a component of prehybridization(4) and hybridization buffer in single-label in situ hybridization
as a carrier to enhance recovery of RNA from small numbers of cells

Biochem/physiol Actions
Transfer RNAs (tRNAs) play an important role in translation. It is responsible for the addition of amino acids to ribosome for peptide chain formation. The tRNA contains an anticodon region for mRNA base pairing and two attachment regions: for amino acid binding and tRNA synthetase recognition. The ribosomal RNA facilitates the movement of tRNA along the mRNA.
Preparation Note
tRNA is provided in a solution in 10 mM Tris HCl (pH 7.4) in 1 mM EDTA.