Merck, SAE0215-10U, Inorganic Pyrophosphatase from Escherichia coli

Name: Inorganic Pyrophosphatase from Escherichia coli
Brand: Merck
SKU: SAE0215-10U

Size:, ≥100 units/mL, buffered aqueous solution

Available Catalog Numbers

SAE0215-10U SAE0215-50U

Catalog Number: SAE0215-10U

Pricing

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Category

Recombinant Enzymes

Pack Size100 units / pack

CollectionsMerck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

General description
Inorganic pyrophosphatase (PPase) is a ubiquitous enzyme that catalyzes pyrophosphate hydrolysis. It plays an important role in energy metabolism by providing a thermodynamic pull for biosynthetic reactions, such as protein, RNA, and DNA synthesis. Nucleic acid synthesis would be energetically impossible in vivo if not coupled with the hydrolysis of pyrophosphate (PPi).
Application
This product is based on the native pyrophosphatase from E. coli, Uniprot No. P0A7A9. Pyrophosphatase in E. coli is a homohexameric protein containing 175 amino acids residues per subunit. This product is a recombinant protein expressed in E. coli and induced by IPTG. Each subunit has a MW of 19.7 kDa and theoretical pI of ~5. The protein activity is Mg2+ dependent and it is a relatively thermostable protein.
Biochem/physiol Actions
Inorganic pyrophosphatase (PPase) is a ubiquitous enzyme that catalyzes pyrophosphate to phosphate. It plays an important role in energy metabolism as it provides a thermodynamic pull for biosynthetic reactions, such as protein, RNA, and DNA synthesis.
Features and Benefits
This product has a purity minimum of 95% (SEC-HPLC) and an activity minimum of 100 units/mL to enhance RNA yield during transcription.
Physical form
The product is supplied as an aqueous solution containing 20mM Tris-HCl, 100mM NaCl, 1mM DTT, 0.1mM EDTA, and 50% glycerol, titrated to pH 8 at 25 °C.
Other Notes
One unit will release 1.0 µmole of inorganic orthophosphate per minute at pH 9 at 25 °C. The reaction buffer used for determination of enzyme activity contains 50 mM Tris-HCl, pH 9.0.

Specifications

General

UNSPSC Code	12352204
NACRES	NA.28
biological source	Escherichia coli
recombinant	expressed in E. coli
grade	Molecular Biology
description	Recombinant, expressed in E.coli
assay	≥95% (size exclusion chromatography)
form	buffered aqueous solution
specific activity	≥100 units/mL
shelf life	2 yr at -20 °C ((retest))
mol wt	19.7 kDa
storage condition	OK to freeze
concentration	≥100 units/mL
color	colorless
optimum pH	9.0 (25 °C)
pH	8.0 (25 °C)
solubility	soluble
suitability	suitable for molecular biology
UniProt accession no.	P0A7A9
application(s)	research use
foreign activity	DNAse, none detectedRNAse, none detectedNickase, none detected
shipped in	dry ice
storage temp.	-10 to -25°C
Quality Level	200

Ordering

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