General description
Bladder cancer is one of the top ten most common malignancies worldwide, and no cure is available for metastatic stages of the disease (1). Bladder cancer generally arises from the inner epithelial layer (urothelium) (1). A suitable bladder tumor model that recapitulates human disease both histologically and behaviorally is essential for evaluating new therapeutic agents and modes of treatment. Rat models more closely parallel human bladder carcinoma than mouse models regarding configuration and progress of the disease (2).
The AY-27 rat bladder tumor cell line is a urothelium-derived transitional cell carcinoma (3) and is the most widely used animal model for bladder cancer. AY-27 cells are tumorigenic and are characterized by low adenylate cyclase activity (4), lack of FasL expression, and positive staining for the urothelial marker cytokeratin-7.5 Tumors derived from AY-27 cells demonstrate orthotopic growth, allowing exposure to anti-tumor drugs in a natural environment (5). The high reproducibility and clinical relevance of the AY-27 tumor model has contributed greatly to the understanding of bladder cancer and evaluation of potential treatments.
References:
1. Sanli O et al. (2017) Bladder cancer. Nat Rev Dis Primers 3: 17022.
2. Oyasu R (1995) Epithelial tumors of the lower urinary tract in humans and rodents. Food Chem Toxicol. 33(9): 747-755.
3. Cohen SM et al. (1981) Transplantation and cell culture of rat urinary bladder carcinoma. Invest Urol. 19(3): 136-141.
4. Chlapowski FJ, Nemecek GM (1985) Aberrant cyclic adenosine 3′:5′-monophosphate metabolism in cultures of tumorigenic rat urothelium. Cancer Res. 45(1): 122-127.
5. Xiao Z et al. (1999) Characterization of a novel transplantable orthotopic rat bladder transitional cell tumor model. Br J Cancer 81(4): 638-646.
Source: The AY-27 cell line was established from a primary bladder tumor induced by N-[4-(5-nitro-2-furyl)-2-thiazolyl]formamide (FANFT) treatment of Fischer F344 rats (2).
Application
Subject to local law, this product is intended to be sold for internal in vitro research use only subject to terms and conditions found here: www.sigmaaldrich.com/restrictedcelluse. This product may not be: re-engineered or copied; used to make derivatives, modifications or functional equivalents; used to obtain patents or other IP claiming use of the product; used to develop, test, or manufacturer a commercial product; used as a component in a commercial product; resold or licensed; used in any clinical applications or trials; or used in humans. A license or limited commercial use agreement is required for use by any for-profit entity, use in services, and use in sponsored academic research. For information regarding any such use, please contact
[email protected].
Preparation Note
Store in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
Analysis Note
Each vial contains >=1X106 viable cells.
Cells are tested negative for infectious diseases by a Mouse/Rat Comprehensive CLEAR panel by Charles River Animal Diagnostic Services.
Cells are verified to be of rat origin and negative for inter-species contamination from mouse, chinese hamster, Golden Syrian hamster, human and non-human primate (NHP) as assessed by a Contamination CLEAR panel by Charles River Animal Diagnostic Services.
Cells are negative for mycoplasma contamination.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
