Merck, SCT122, GelRed® Nucleic Acid Stain 10000X DMSO

Name: GelRed® Nucleic Acid Stain 10000X DMSO
Brand: Merck
SKU: SCT122

Size:0.5 mL, Fluorescent DNA gel stain, GelRed is a fluorescent nucleic acid stain designed to replace the highly toxic ethidium bromide (EtBr) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels.

Catalog Number: SCT122

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Category

Nucleic Acid Stains

Pack Size0.5 mL

TagsMolecular Biology & Functional Genomics Nucleic Acid Electrophoresis & Hybridization Nucleic Acid Electrophoresis Transfer & Quantitation

CollectionsMerck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

General description
GelRed is a sensitive, stable and environmentally safe fluorescent nucleic acid dye designed to replace the highly toxic ethidium bromide (EtBr) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels. GelRed and EtBr have virtually the same spectra (Figure 1), so you can directly replace EtBr with GelRed without changing your existing imaging system. In addition, GelRed is far more sensitive than EtBr (Figure 2).

The dye is noncytotoxic and non-mutagenic at concentrations well above the working concentrations used in gel staining. GelRed successfully passed environmental safety tests in compliance with CCR Title 22 Hazardous Waste Characterization, under which GelRed is not classified as hazardous waste.

Spectral Properties
Absorbance: Standard Transilluminator (302 or 312 nm)
Emission: Ethidium Bromide, SYBR or GelStar Filter
Application
GelRed is a fluorescent nucleic acid stain designed to replace the highly toxic ethidium bromide (EtBr) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels.
Nucleic acid detection
Research Category
Cell Culture
Research Sub Category
Live Cell Dye
Physical form
Liquid
Preparation Note
GelRed is a very stable dye. Store GelRed at room temperature, protected from light. Dye precipitation may occur at lower temperatures, resulting in lower signal or the appearance of precipitate on the surface of the gel. If this occurs, heat the solution to 45-50°C for two minutes and vortex. Protect From Light.
Analysis Note

Spectral Properties
Absorbance: Standard Transilluminator (302 or 312 nm)
Emission: Ethidium Bromide, SYBR or GelStar Filter
Legal Information
GelRed is a registered trademark of Biotium Inc.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Specifications

General

NACRES	NA.31
UNSPSC Code	12352106
technique(s)	PCR: suitable
web metadata keyword.default	microbialanalysismicrobialanalysis
detection method	fluorometric

FAQs

Question: What is the stock concentration and package size?

Answer:GelRed is supplied as a 10,000X concentrate in DMSO, in a 0.5 mL package.

Question: Which nucleic acids can the fluorescent stain detect?

Answer:The stain can visualize double-stranded DNA, single-stranded DNA and RNA in electrophoresis gels.

Question: How should GelRed concentrate be stored?

Answer:Store at room temperature away from light; cold storage may cause dye precipitation.

Application Note

Merck GelRed is a concentrated fluorescent nucleic acid stain for visualizing DNA and RNA after electrophoretic separation. The 10,000X stock is supplied in DMSO in a 0.5 mL format and can be diluted for agarose or polyacrylamide gel staining workflows. GelRed supports imaging of double-stranded DNA, single-stranded DNA and RNA with ultraviolet transillumination near 302–312 nm and optical filter sets normally used for ethidium bromide, SYBR or GelStar detection. It is designed as an alternative to conventional ethidium bromide staining, although all laboratory dyes still require suitable chemical handling and disposal. Users can employ precast or post-staining protocols where validated for their gel systems. Dye concentration, gel thickness and exposure settings affect band brightness and background. Store the concentrated stain at room temperature protected from light; cooling may cause precipitation that should be resolved before preparing a working stain.

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