Merck, SRP3283-50UG, FETUIN A human

General description
The gene AHSG (α-2-HS-glycoprotein, also referred to as fetuin-A) is mapped to human chromosome 3q27. Fetuin A/AHSG is a human plasma glycoprotein belonging to the Cystatin family of proteases inhibitors. Fetuin A is highly expressed in embryonic cells and adult hepatocytes, and is expressed to a lesser extent in monocytes/macrophages. The protein has cystatin-like domains, a calcium phosphate-binding site and a TGF (transforming growth factor)-β cytokine-binding motif. Recombinant human fetuin-A is a heterodimeric protein containing a 282 amino acid A chain and a 27 amino acid B chain. Due to glycosylation, recombinant fetuin-A migrates at an apparent molecular weight of 45–55kDa by SDS-PAGE run under nonreducing conditions.
Application
FETUIN A human has been used to stimulate mRNA expression in human monocytic THP1 cells, human in vitro differentiated adipocytes and C57BL/6 mice.
Biochem/physiol Actions
AHSG (α-2-HS-glycoprotein, also referred to as fetuin-A) is a major serum protein component that exerts various calcium dependent physiological activities, and can mediate growth signaling in certain tumor cells. It also can act as a natural antagonist against specific TGF-β (transforming growth factor β) and BMP (bone morphogenetic protein) signaling proteins. High levels of fetuin-A in the serum is associated with mild osteogenesis imperfecta. On the other hand, reduced levels are seen in Paget′s disease. It is predicted as a potential biomarker for the early diagnosis of hypopharyngeal squamous cell carcinoma.
Physical form
Lyophilized from 10 mM Sodium Phosphate, pH 7.8.
Preparation Note
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.