Phenomenex, 00D-4254-Y0, Luna 3 µm CN 100 Å, LC Column 100 x 3 mm, Ea

The Phenomenex Luna CN analytical column contains a cyanopropyl bonded stationary phase and is listed for normal-phase liquid chromatography. Its 100 × 3.0 mm format uses 3 µm particles with 100 Å pores, providing a distinct separation option for compounds whose polar interactions are important to resolution. Cyanopropyl functionality has different selectivity from bare silica, C18 and phenyl phases and can be evaluated for isomeric or structurally related small molecules. In normal-phase method development, the choice of organic solvent system and sample diluent strongly influences analyte retention and peak symmetry. Use solvents that are mutually compatible and avoid abrupt changes between highly dissimilar mobile-phase compositions. The column should be equilibrated thoroughly before recording retention times, and the sample should be clarified to protect the packed bed. Select operating conditions using the column chemistry and intended instrument limits. This is an analytical USP L10 cyano column, not a C18 reversed-phase column or a hollow chromatographic tube.