Phenomenex, 00F-4753-E0, Luna Omega 5 µm PS C18 100Å, LC Column 150 x 4.6 mm, EA
Catalog Number: 00F-4753-E0
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| Length (mm) | 150.0000 mm |
|---|---|
| Particle Size | 5.0000 µm |
| Internal Diameter | 4.6000 mm |
| Film Thickness | 0.0000 µm |
Answer:PS refers to a positively charged surface that provides additional polar and ionic selectivity alongside C18 hydrophobic retention.
Answer:Polar acidic analytes may show increased retention, while some basic compounds may exhibit improved peak shape.
Answer:The column is 150 × 4.6 mm and contains 5 µm particles with 100 Å pores.
The Phenomenex Luna Omega PS C18 column provides mixed hydrophobic and polar/ionic selectivity within a 150 × 4.6 mm reversed-phase HPLC format. Its 5 µm, 100 Å silica particles have C18 ligands and a positively charged surface functionality. The C18 component promotes retention of nonpolar compounds, while the positive surface increases retention of many acidic analytes and can improve peak shape for basic molecules through ionic repulsion. This selectivity is valuable when conventional C18 phases offer insufficient retention or resolution of polar acids. The stationary phase is compatible with fully aqueous mobile phases, enabling gradients to begin at very low organic-solvent content. Optimize mobile-phase pH and ionic strength to control the relative polar and hydrophobic interactions. Equilibrate the column, prevent particulate contamination and verify sample recovery before routine analysis. This is an analytical column, not the neutral-surface Luna Omega C18 phase.
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