Proteintech, 10001-0-AP, 6*His, His-tag Polyclonal antibody

10001-0-AP targets 6*His, His-tag in WB, IHC, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with recombinant protein samples.
Tested Applications
Positive WB detected in: recombinant protein
Recommended dilution
Western Blot (WB): WB : 1:500-1:2000
Background Information
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The antibody reacts to the 6*His tagged protein.