Send an RFQ for Proteintech srbAF488-1-100ul, ChromoTek Nano-Secondary® anti-human IgG/anti-rabbit IgG, recombinant VHH, Alexa Fluor® 488 [CTK0101, CTK0102]. Iright supports global sourcing and international shipment for life science and laboratory products.
| Description | Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH reagent uses a novel class of anti-rabbit IgG and anti-human IgG specific antibodies. This secondary antibody product consists of Nanobodies that bind to rabbit IgG and human IgG with high affinity & specificity. Nano-Secondary reagents enable simultaneous incubation with primary antibodies, thus shortening your experimental. Due to their small size, Nano-Secondary reagents provide enhanced image resolution. No cross-reactivity against serum proteins from other species, details see below. |
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| Applications | IF, WB, FC |
| Host | Alpaca |
| Species Reactivity | Rabbit, Human, Macaque No cross-reactivity to mouse, rat, sheep, goat, and guinea pig IgG |
| Target/Specificity | Fc-fragment of human IgG, Fab- and Fc-fragment of rabbit IgG (co-reactivity) |
| Physical State | Liquid |
| Conjugate | Alexa Fluor® 488 |
| Suggested Dilution | Immunofluorescence 1:1,000 Super-resolution microscopy 1:1,000 Western blot 1:1,000 |
| Purification Method | Recombinant expression, affinity purification IMAC |
| Type | Mixture of 2 monoclonal Nanobodies; Secondary Nanobody |
| Class | Recombinant |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| Format | Alpaca single domain antibody, monovalent |
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| Cross-reactivity | No cross-reactivity to goat, guinea pig, mouse, rat, and sheep serum Cross-reactivity to macaque serum |
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| Immunogen | Purified rabbit IgG |
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| Clonality | Biclonal: mixture of 2 monoclonal Nanobodies |
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| Clones | CTK0101 (VHH0244): Fc-fragment of human IgG, Fc-fragment of rabbit IgG CTK0102 (VHH0245): Fab-fragment of rabbit IgG |
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| Affinity (KD) | CTK0101: K D = 0.2 nM, CTK0102: K D = 1.2 nM |
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| RRID | AB_2827585 |
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| Excitation / Emission | Excitation max: 490 nm, Emission max: 525 nm |
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| Storage Buffer | 10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09 % Sodium azide |
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| Storage Condition | Store at -20°C short term or -80°C long term. Aliquot upon delivery. Avoid freeze-thaw cycles. |
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| Size | 10 μL; 100 μL |
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| Note | This product is for research use only, not for diagnostic or therapeutic use |
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