Proteintech, 65566-1-MR, Anti-Human CD107b / LAMP2 (H4B4) Mouse IgG2a Recombinant Antibody

65566-1-MR targets CD107b / LAMP2 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.
Tested Applications
Positive IF/ICC detected in: HeLa cells
Positive FC (Intra) detected in: Jurkat cells
Recommended dilution
Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.5 ug per 10^6 cells in 100 μl suspension
Background Information
LAMP2 (CD107b) is a Lysosomal membrane glycoprotein. LAMP2 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP-2 is localized primarily in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane under certain circumstances, e.g., after platelet activation, during granulocytic differentiation and activation, and in some tumor cells (PMID: 12221139). LAMP is involved in lysosomal stability and autophagy (PMID: 12221139). This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may play a role in tumor cell metastasis (PMID 9426697).