Proteintech, 65596-1-MR, Anti-Human CD107a / LAMP1 (H4A3) Mouse IgG2a Recombinant Antibody

65596-1-MR targets CD107a / LAMP1 in IF/ICC, FC, FC (Intra) applications and shows reactivity with human samples.
Tested Applications
Positive IF/ICC detected in: HepG2 cells
Positive FC (Intra) detected in: Jurkat cells
Positive FC detected in: Thrombin treated human peripheral blood platelets
Recommended dilution
Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in 100 μl suspension
Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension
Background Information
LAMP1 (CD107a) is a heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP1 is expressed largely in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane (PMID: 16168398). Elevated LAMP1 expression at the cell surface has also been detected during platelet and granulocytic cell activation, as well as in some tumor cells (PMID: 29085473). LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis (PMID: 18835598; 29085473; 9426697).