Proteintech, 65611-1-MR, Anti-Human ALCAM (3A6) Mouse IgG2a Recombinant Antibody

65611-1-MR targets ALCAM in IF/ICC, FC applications and shows reactivity with human samples.
Tested Applications
Positive IF/ICC detected in: hTERT-RPE1 cells
Positive FC detected in: PHA treated human PBMCs
Recommended dilution
Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension
Background Information
Activated leukocyte cell adhesion molecule (ALCAM, also known as CD166) is a cell adhesion molecule that belongs to the immunoglobulin superfamily. It is involved in cell-cell adhesion through homophilic and heterophilic (to CD6) interactions. ALCAM is widely expressed in a variety of normal tissues and cell types, including activated T cells and monocytes, epithelial cells, fibroblasts, neuronal cells, hepatocytes, and bone marrow mesenchymal stem cells (PMID: 7760007; 25221999). Altered ALCAM expression has been associated with the differentiation state and progression in some neoplasms including melanoma, prostate, colorectal, and breast cancers (PMID: 20461761; 18172759).