Proteintech, 84814-1-RR, 6*His, His-Tag Recombinant monoclonal antibody

84814-1-RR targets 6*His, His-Tag in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with recombinant protein samples.
Tested Applications
Positive WB detected in: Transfected HEK-293T cells
Positive IF/ICC detected in: HEK-293 cells
Positive FC (Intra) detected in: Transfected HEK-293T cells
Recommended dilution
Western Blot (WB): WB : 1:1000-1:6000
Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
Background Information
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The antibody reacts to the 6*His tagged protein.