Proteintech, 98057-1-RR, Anti-Human CD126/IL-6R alpha Rabbit Recombinant Antibody

98057-1-RR targets CD126/IL-6R alpha in FC applications and shows reactivity with human samples.
Tested Applications
Positive FC detected in: human PBMCs
Recommended dilution
Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension
Background Information
Interleukin-6 (IL-6) is a pleiotropic cytokine produced by a variety of cells during infection, trauma, and immunological challenge (PMID: 8274730). IL-6 acts via a receptor complex consisting of two distinct membrane-bound glycoproteins, an 80-kDa IL-6-binding subunit (IL-6R alpha, CD126, gp80) and a 130-kDa signal-transducing element (gp130, CD130). After binding of IL-6 to membrane-bound IL-6R alpha, the complex of IL-6 and IL-6R alpha associates with gp130, thus activating the receptor (PMID: 9716487). Expression of gp130 is found in almost all organs while expression of IL-6R alpha is predominantly confined to hepatocytes and leukocyte subpopulations (monocytes, neutrophils, T cells, and B cells) (PMID: 11149892). Soluble form of IL-6R alpha has been found in human serum and urine (PMID: 2529343; 1545125).