Proteintech, 98262-1-RR, Anti-GGGGS Linker Rabbit Recombinant Antibody

98262-1-RR targets GGGGS Linker in WB, IHC, IF/ICC, FC applications and shows reactivity with recombinant protein samples.
Tested Applications
Positive WB detected in: Transfected HEK-293T cells
Positive IHC detected in: Transfected HEK-293T cellsNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0
Positive IF/ICC detected in: Transfected HEK-293T cells, Transfected CHO-K1
Positive FC detected in: Transfected HEK-293T cells
Recommended dilution
Western Blot (WB): WB : 1:1000-1:6000
Immunohistochemistry (IHC): IHC : 1:40000-1:160000
Immunofluorescence (IF)/ICC: IF/ICC : 1:2000-1:8000
Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension
Background Information
As a crucial element in the design of recombinant fusion proteins, linkers play an increasingly vital role in the construction of stable, bioactive fusion proteins (PMID: 23026637). GGGGS linker (G4S linker) is a flexible linker made of 4 glycine repeats followed by a serine amino acid (PMID:3045807). Due to its flexibility and resistance to proteases, GGGGS and its repeats are commonly used when engineering a protein, particularly in the construction of single-chain Fv (ScFv) domains expressed on the surfaces of CAR-T cells (PMID:23581628; 36874404). This antibody, raised against a synthetic peptide (GGGGSGGGGSGGGGS), can recognize G4S*2, G4S*3, G4S*4, and G4S*5.