Proteintech, 98616-1-RR, Anti-Human CD15 Rabbit Recombinant Antibody

98616-1-RR targets CD15 in IHC, FC applications and shows reactivity with human samples.
Tested Applications
Positive IHC detected in: human tonsillitis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0
Positive FC detected in: human peripheral blood leukocytes
Recommended dilution
Immunohistochemistry (IHC): IHC : 1:50-1:500
Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension
Background Information
CD15, also known as stage-specific embryonic antigen-1 (SSEA-1) or Lewis X (LeX), is a cell surface glycan consisting of a trisaccharide with the structure Galβ1-4[Fucα1-3]GlcNAc (PMID: 18953356). CD15 is expressed on most terminally differentiated myeloid cells, including neutrophils, eosinophils, monocytes, macrophages, mast cells, and Langerhans cells. It has also been shown to be expressed on stem cells and progenitors in the embryonic and adult nervous system, Hodgkin's lymphoma cells, and cells from a variety of solid cancers (PMID: 19185848). CD15 acts as a ligand for selectins in transendothelial migration and is involved in phagocytosis and chemotaxis (PMID: 1362195).