Qiagen, 19065, Buffer PE (concentrate, 100 mL)

Name: Buffer PE (concentrate, 100 mL)
Brand: Qiagen
SKU: 19065

100 mL Wash Buffer (5x concentrate for 500 mL buffer)

Catalog Number: 19065

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Category

Plasmid Purification Kits

Pack Size100 mL

TagsDiscovery & Translational Research DNA & RNA Purification DNA Plasmid DNA

Description

The QIAsprint workflow for plasmid DNA isolation from bacteria is a modular magnetic bead system enabling high-throughput plasmid preps with minimal manual interaction. After pretreatment, samples are loaded onto the QIAsprint Connect instrument for automated purification. The system combines modular flexibility with proven magnetic bead technology to support scalable processing of transfection-grade plasmid DNA. The workflow consists of dedicated modules that can be flexibly combined. You can start directly from bacterial culture using a walk-away protocol or from pelleted bacteria. In both cases, BOPP technology eliminates the need for lysate clearing by centrifugation or buffer pipetting steps. QIAsprint Plasmid BOPP PrepSet (384) This module is tailored for extraction pretreatment and enables the “beads-only” plasmid workflow. The patent-pending BOPP technology allows lysate clearing without centrifugation. The PrepSet includes Buffer P1, P2 and P3, Buffer QBB, Buffer ETR2 and RNase A. QIAsprint Essential Kit D (384) Used in combination with the QIAsprint Plasmid BOPP PrepSet, this module is recommended for purification of plasmid DNA from bacteria. The Essential Kit provides the magnetic beads and wash buffers required for high-yield, reproducible purification of high-quality, transfection-grade plasmid DNA. The kit includes Buffer PE, EB and MagAttract Suspension G. QIApHish Beads (384) QIApHish Beads enable direct capture of bacterial cells from liquid culture. Combined with the QIAsprint Plasmid BOPP PrepSet and Essential Kit D, they support automated plasmid DNA purification with consistent yield and reproducibility. All modules are optimized for automated processing of up to 384 samples on the QIAsprint Connect, ensuring minimal hands-on time and reproducible performance at high throughput.
The QIAsprint Plasmid BOPP PrepSet workflow for plasmid DNA isolation from bacteria delivers exceptional speed and high processing performance, enabling automated nucleic acid extraction with minimal manual intervention (see Processing time). Flexible workflow options allow either direct processing from bacterial cultures using a true walk-away protocol or starting from pelleted bacteria. In both cases, BOPP technology eliminates the need for lysate clearing by centrifugation (see QIAsprint plasmid DNA workflow).
The QIAsprint workflow for plasmid DNA isolation from bacterial cells uses a modular approach that enables an efficient culture-to-eluate protocol. The procedure supports automated processing directly from bacterial cultures or from pelleted bacteria. When starting from culture, QIApHish Beads capture bacterial cells directly, eliminating the need for centrifugation-based harvesting (see QIAsprint plasmid DNA workflow). During pretreatment, the QIAsprint Plasmid BOPP PrepSet applies the BOPP technology to clear lysates without centrifugation or manual transfer steps. This streamlined pretreatment integrates directly with the automated purification workflow, significantly reducing hands-on time while maintaining consistent performance. The workflow recovers ready-to-use, transfection-grade plasmid DNA from up to 1 mL bacterial cultures when using the Culture protocol or from up to 5 mL bacterial cultures when using the Pellet protocol. The approach is suitable for both high-copy and low-copy plasmids. In addition, most buffers and reagents used within the workflow are available as standalone components, enabling further customization for specialized applications (see QIAsprint sets and consumables finder).
Workflow A: Plasmid purification directly from bacterial cultures This workflow includes capture of bacterial cells, lysis, binding, washing and elution. The pH of the bacterial culture is adjusted and QIApHish Beads are added to capture bacterial cells. The bead-bound cells are transferred to perform lysis, neutralization and lysate clearing. MagAttract Suspension is then added to bind plasmid DNA from the cleared lysate, followed by wash and elution steps to yield ready-to-use plasmid DNA (see QIAsprint Plasmid Workflow). Workflow B: Plasmid purification from pelleted bacterial cultures This workflow includes lysis, binding, washing and elution. Bacterial cultures are pelleted and resuspended before lysis, neutralization and lysate clearing are performed on the QIAsprint Connect. MagAttract Suspension binds plasmid DNA from the cleared lysate, followed by wash and elution steps to yield ready-to-use plasmid DNA (see QIAsprint Plasmid Workflow).

Specifications

General

Elution volume	Variable, depending on sample: 50 µL and 100 µL recommended
Main sample type	Plasmid carrying E. coli grown in LB medium
Processing	Automated on QIAsprint
Analyte	Plasmid DNA
Sample amount	Up to 1 mL bacterial culture (culture protocol), up to 5 mL bacterial culture (pellet protocol)
Technology	Magnetic-particle technology

FAQs

Question: Is Buffer PE ready to use straight from the bottle?

Answer:No. The 100 mL bottle contains 5× wash-buffer concentrate and yields 500 mL of prepared buffer after dilution as required by the relevant QIAGEN protocol.

Question: Which purification step uses Buffer PE?

Answer:Buffer PE washes the bound DNA on silica membranes after binding and before elution.

Question: Can Buffer PE replace Buffer EB?

Answer:No. Buffer PE is a wash buffer, whereas Buffer EB is an elution buffer for recovering purified nucleic acids.

Application Note

QIAGEN Buffer PE is a concentrated wash buffer used to remove residual impurities from silica-membrane DNA purification columns. The 100 mL bottle contains a fivefold concentrate that produces 500 mL of working wash buffer after the prescribed dilution. Buffer PE is used in QIAquick and MinElute DNA cleanup procedures, including workflows that recover DNA from PCR reactions or agarose gels. In a typical spin-column sequence, DNA binds to the membrane, impurities are washed away with prepared Buffer PE, and purified DNA is subsequently eluted with a low-salt elution solution. Proper preparation of the wash buffer before first use is essential for reliable results. A dry-spin step after washing helps prevent residual wash liquid from carrying into downstream enzymatic reactions. Buffer PE is a wash reagent, not an elution buffer or DNA-binding reagent.

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