Qiagen, 19086, Buffer EB (250 mL)

Name: Buffer EB (250 mL)
Brand: Qiagen
SKU: 19086

250 mL Elution Buffer

Catalog Number: 19086

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Category

Plasmid Purification Kits

Pack Size250 mL

TagsDiscovery & Translational Research DNA & RNA Purification DNA Plasmid DNA

Description

The QIAsprint workflow for plasmid DNA isolation from bacteria is a modular magnetic bead system enabling high-throughput plasmid preps with minimal manual interaction. After pretreatment, samples are loaded onto the QIAsprint Connect instrument for automated purification. The system combines modular flexibility with proven magnetic bead technology to support scalable processing of transfection-grade plasmid DNA. The workflow consists of dedicated modules that can be flexibly combined. You can start directly from bacterial culture using a walk-away protocol or from pelleted bacteria. In both cases, BOPP technology eliminates the need for lysate clearing by centrifugation or buffer pipetting steps. QIAsprint Plasmid BOPP PrepSet (384) This module is tailored for extraction pretreatment and enables the “beads-only” plasmid workflow. The patent-pending BOPP technology allows lysate clearing without centrifugation. The PrepSet includes Buffer P1, P2 and P3, Buffer QBB, Buffer ETR2 and RNase A. QIAsprint Essential Kit D (384) Used in combination with the QIAsprint Plasmid BOPP PrepSet, this module is recommended for purification of plasmid DNA from bacteria. The Essential Kit provides the magnetic beads and wash buffers required for high-yield, reproducible purification of high-quality, transfection-grade plasmid DNA. The kit includes Buffer PE, EB and MagAttract Suspension G. QIApHish Beads (384) QIApHish Beads enable direct capture of bacterial cells from liquid culture. Combined with the QIAsprint Plasmid BOPP PrepSet and Essential Kit D, they support automated plasmid DNA purification with consistent yield and reproducibility. All modules are optimized for automated processing of up to 384 samples on the QIAsprint Connect, ensuring minimal hands-on time and reproducible performance at high throughput.
The QIAsprint Plasmid BOPP PrepSet workflow for plasmid DNA isolation from bacteria delivers exceptional speed and high processing performance, enabling automated nucleic acid extraction with minimal manual intervention (see Processing time). Flexible workflow options allow either direct processing from bacterial cultures using a true walk-away protocol or starting from pelleted bacteria. In both cases, BOPP technology eliminates the need for lysate clearing by centrifugation (see QIAsprint plasmid DNA workflow).
The QIAsprint workflow for plasmid DNA isolation from bacterial cells uses a modular approach that enables an efficient culture-to-eluate protocol. The procedure supports automated processing directly from bacterial cultures or from pelleted bacteria. When starting from culture, QIApHish Beads capture bacterial cells directly, eliminating the need for centrifugation-based harvesting (see QIAsprint plasmid DNA workflow). During pretreatment, the QIAsprint Plasmid BOPP PrepSet applies the BOPP technology to clear lysates without centrifugation or manual transfer steps. This streamlined pretreatment integrates directly with the automated purification workflow, significantly reducing hands-on time while maintaining consistent performance. The workflow recovers ready-to-use, transfection-grade plasmid DNA from up to 1 mL bacterial cultures when using the Culture protocol or from up to 5 mL bacterial cultures when using the Pellet protocol. The approach is suitable for both high-copy and low-copy plasmids. In addition, most buffers and reagents used within the workflow are available as standalone components, enabling further customization for specialized applications (see QIAsprint sets and consumables finder).
Workflow A: Plasmid purification directly from bacterial cultures This workflow includes capture of bacterial cells, lysis, binding, washing and elution. The pH of the bacterial culture is adjusted and QIApHish Beads are added to capture bacterial cells. The bead-bound cells are transferred to perform lysis, neutralization and lysate clearing. MagAttract Suspension is then added to bind plasmid DNA from the cleared lysate, followed by wash and elution steps to yield ready-to-use plasmid DNA (see QIAsprint Plasmid Workflow). Workflow B: Plasmid purification from pelleted bacterial cultures This workflow includes lysis, binding, washing and elution. Bacterial cultures are pelleted and resuspended before lysis, neutralization and lysate clearing are performed on the QIAsprint Connect. MagAttract Suspension binds plasmid DNA from the cleared lysate, followed by wash and elution steps to yield ready-to-use plasmid DNA (see QIAsprint Plasmid Workflow).

Specifications

General

Elution volume	Variable, depending on sample: 50 µL and 100 µL recommended
Main sample type	Plasmid carrying E. coli grown in LB medium
Processing	Automated on QIAsprint
Analyte	Plasmid DNA
Sample amount	Up to 1 mL bacterial culture (culture protocol), up to 5 mL bacterial culture (pellet protocol)
Technology	Magnetic-particle technology

FAQs

Question: What is the composition of Buffer EB?

Answer:Buffer EB contains 10 mM Tris-Cl at pH 8.5.

Question: What is Buffer EB used for?

Answer:It elutes purified nucleic acids from silica-based purification columns after the washing steps are complete.

Question: Does Buffer EB contain EDTA?

Answer:No. Buffer EB is a Tris-based elution buffer without EDTA, which can be useful for EDTA-sensitive downstream reactions.

Application Note

QIAGEN Buffer EB is a low-salt elution buffer for recovering purified DNA and other nucleic acids from silica-based purification procedures. Its formulation is 10 mM Tris-Cl at pH 8.5, allowing DNA to be released from a washed silica membrane into a buffered solution. The 250 mL bottle is suitable for repeated elution steps in QIAprep, QIAquick, MinElute, AllPrep and QIAGEN Plasmid Plus workflows where Buffer EB is specified. In spin-column purification, Buffer EB is applied after completion of the wash and dry-spin steps. Elution efficiency depends on the membrane, sample amount, elution volume and protocol conditions. The EDTA-free formulation can be advantageous for downstream enzymatic applications where chelating agents might interfere with magnesium-dependent enzymes. Buffer EB is an elution reagent; it is not a lysis, binding or membrane-wash solution.

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