Description
The QIAprep Spin Miniprep Kit is designed for isolating high-purity plasmid or cosmid DNA, offering up to 20 µg yields for various molecular biology applications like sequencing and cloning. Higher yields (up to 30 µg) can be achieved using the High-Yield Supplementary Protocol. The kit is automatable on the QIAcube Connect. Want to try the QIAprep Spin Miniprep Kit for the first time? Request a quote for a trial kit. For optimal results, we recommend pairing this kit with the QIAvac 24 Plus system.
The QIAprep Spin Miniprep Kit or QIAwave Plasmid Miniprep Kit enables purification of up to 20 µg molecular biology grade plasmid DNA or cosmid DNA for use in routine molecular biology applications such as PCR, sequencing and cloning. Versatile QIAprep 2.0 Spin Columns can be used either in microcentrifuges, on vacuum manifolds, or in the QIAcube (see figures "QIAprep 2.0 Spin Column handling options A, B, and C"). The vacuum procedure provides simplified handling and faster sample processing. QIAprep 2.0 Spin Columns can be vacuum processed using the QIAvac 24 Plus or any other commercial manifold with luer connectors. The QIAprep Spin Miniprep Kit can now also be fully automated on the QIAcube Connect (see figure "QIAcube Connect"). The QIAwave Plasmid Miniprep Kit and QIAprep Spin Miniprep Kit deliver identical performance due to similar chemistry. Comparative data show that both kits have superior performance over competitor kits (see figure "QIAwave Kit performance"). QIAprep Spin and QIAwave Plasmid Miniprep Kit Format Spin columns Purification module QIAprep 2.0 Spin Columns Throughput 1–24 samples Preparation time 24 minipreps in 30 minutes Equipment required Microcentrifuge or vacuum manifold; fully automatable using the QIAcube Connect Lysate clearing Centrifugation Capacity of column reservoir 800 µl Minimum elution buffer volume 50 µl Culture volume for high-copy plasmids 1–5 ml Culture volume for low-copy plasmids/cosmids 1–10 ml Purified DNA can be used in restriction digestion (see figure "Complete digestion with various restriction enzymes").
QIAprep 2.0 Spin Columns contain a unique silica membrane that binds up to 20 µg DNA in the presence of a high concentration of chaotropic salt, and allows elution in a small volume of low-salt buffer. QIAprep membrane technology eliminates time consuming phenol-chloroform extraction and alcohol precipitation, as well as the problems and inconvenience associated with loose resins and slurries. High-purity plasmid DNA eluted from QIAprep 2.0 Spin Columns is immediately ready to use – there is no need to precipitate, concentrate, or desalt. To enable faster and more convenient sample processing and analysis, gel loading dye is provided in the kit. GelPilot Loading Dye contains 3 tracking dyes (xylene cyanol, bromophenol blue, and orange G) to facilitate the optimization of agarose gel run time and prevent smaller DNA fragments migrating too far (see figure "GelPilot Loading Dye"). For a more sustainable alternative, we have the QIAwave Plasmid Miniprep Kit. This kit reduces plastic and cardboard usage by up to 22% and 14%, respectively. It features waste tubes made from 100% recycled plastic, which can be reused throughout the procedure. The QIAwave buffers, in concentrated form, cut down plastic consumption by up to 93% per bottle. Despite the visual difference, the QIAwave Kit maintains user-friendliness with chemistry and performance identical to the standard kit. Please be aware that you will need sterile glass bottles to store the reconstituted buffers. In partnership with My Green Lab, we've also assessed the environmental impact of the QIAprep Spin Miniprep Kit (50/250) and QIAwave Plasmid Miniprep Kit (50/250). My Green Lab ACT labels are designed to evaluate and score products on several sustainability criteria. These include: • Manufacturing • Responsible chemical management • Sustainable content within products and packaging materials • Disposal of the packaging at the end of life Products are scored from 1 to 10 except for energy and water consumption, which are scored as 1 point per kWh or gallon, respectively. A low score means a lower environmental impact – (see figures"„Plasmid Miniprep Kits ACT environmental impact factor label US (50 / 250), EU (50 / 250) and UK (50 / 250).”
Plasmid purification using QIAprep Kits and the QIAwave Plasmid Miniprep Kit follows a simple bind-wash-elute procedure (see flowchart "The QIAprep procedure"). First, bacterial cultures are lysed and the lysates are cleared by centrifugation. The cleared lysates are then applied to the QIAprep 2.0 module where plasmid DNA adsorbs to the silica membrane. Impurities are washed away and pure DNA is eluted in a small volume of elution buffer or water. In addition to plasmid purification from Escherichia coli, QIAprep Kits can be used to purify plasmid DNA from Saccharomyces cerevisiae, Bacillus subtilis, and Agrobacterium tumefaciens. Contact QIAGEN Technical Services or your local distributor for protocols for these applications. To enhance your experience, standard protocols can be executed using the TRACKMAN Connected system, combined with PIPETMAN M Connected pipettes, both from Gilson. This system not only guides researchers through protocols but also automatically adjusts Bluetooth-enabled pipette settings. Download more information.
