Answer:It supplies fluorescent internal sizing peaks for capillary electrophoresis fragment analysis, supporting base-pair assignment of STR amplicons.
Answer:No. A size standard calibrates fragment lengths; an allelic ladder supplies known STR allele references for genotype designation.
Answer:The package supports 100 reactions and uses the BTO fluorescent dye channel. The genetic analyzer's dye calibration must be compatible.
QIAGEN DNA Size Standard 550 (BTO) is a fluorescent internal size reference for capillary electrophoresis fragment analysis in forensic human-identification workflows. The BTO-labeled standard provides migration reference peaks that allow compatible genetic analysis software to assign base-pair sizes to amplified STR fragments. It is used with selected Investigator STR assay configurations and appropriate dye-channel calibration settings, where accurate fragment sizing is essential for interpreting allelic peaks. The 100-reaction package is a size-standard reagent rather than an allelic ladder, PCR amplification kit or conventional agarose-gel DNA ladder. Include the specified amount of size standard in each prepared electrophoresis sample, together with the required formamide or other sample-preparation components for the validated workflow. The instrument's spectral calibration must include the BTO dye channel; an unrelated dye-labeled standard cannot be substituted solely because it has a similar size range.
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