Description
The QIAamp 96 DNA QIAcube HT Kit enables simple, automated purification of total DNA (e.g., genomic, mitochondrial, and pathogen) from blood, cells, and tissue samples. Using proven QIAamp silica-membrane technology in a convenient 96-well format, contaminants and inhibitors are removed to yield high-quality nucleic acids that are ready for downstream analysis. This kit is designed to be used on QIAcube HT System.
The QIAamp 96 DNA QIAcube HT Kit provides fast and reliable automated purification of total DNA in 96-well format. Results are highly reproducible, with no significant well-to-well variation in DNA recovery (see figure Reproducible 96-well DNA purification). Automated purification of total DNA from blood samples is quick, easy, and reliable with the QIAamp 96 DNA QIAcube HT Kit. Fresh or frozen whole blood containing common anticoagulants can be processed, and the resulting DNA is of the same high quality as from other QIAGEN purification solutions (see figures Agarose gel of purified genomic DNA and Analysis of genomic DNA from human blood). QIAcube HT and the dedicated QIAamp 96 DNA QIAcube HT Kit lets users increase sample purification throughput without having to compromise on quality or reliability. The procedure provides high yields of pure DNA that perform well in downstream analyses, similar to other QIAGEN DNA purification solutions (see figures High performance in downstream assays and Purification of genomic DNA from tissues).
The QIAamp 96 DNA QIAcube HT Kit enables automated purification of total DNA (e.g., genomic, mitochondrial, and pathogen) from a variety of sample sources including fresh or frozen tissues, cells, and blood. Samples treated with common anticoagulants such as citrate, heparin, or EDTA are suitable for use. The procedure yields high-quality DNA that performs well in PCR and other enzymatic reactions. The QIAamp 96 DNA QIAcube HT Kit combines the selective binding properties of a silica-based membrane with a high-throughput 96-well format, and is designed for fully automated, simultaneous processing of 24–96 samples on the QIAcube HT instrument. Kit specifications Specification Description Number of samples 24–96 samples (to be processed in increments of 8) Sample input volume Blood: up to 200 μl (for sample volumes less than 200 μl, add PBS) Cells: up to 5 x 10 6 cells Tissues: up to 20 mg tissue (10 mg for spleen, liver, or lung tissue) Elution volume 200 μl Duration 96 samples in approximately 96 minutes 24 samples in approximately 45 minutes
The QIAamp 96 DNA QIAcube HT procedure is fast and simple with the QIAcube HT instrument. Samples are first lysed using QIAGEN Proteinase K. Buffering conditions are adjusted to provide optimal DNA binding conditions and the lysate is loaded onto the QIAamp 96 plate. As vacuum is applied, DNA selectively binds to the QIAamp membrane, and contaminants pass through. Remaining contaminants and enzyme inhibitors are removed in three efficient wash steps. Pure DNA is eluted under vacuum in a single step in 200 µl of Buffer AE.
