Description
The QIAamp Virus BioRobot MDx Kit provides automated viral nucleic acid purification on the BioRobot MDx using proven QIAamp technology. The fully automated procedure requires less than 2.5 hours, including bar code reading and complete process documentation, with no hands-on time during the run.
The QIAamp Virus BioRobot MDx process provides uniform DNA and RNA recovery and purity across the 96-well plate with no detectable cross-contamination between samples. Up to 265 µl of cell-free body fluids can be processed. The typical elution volume is 75 µl. Viruses can be detected with high sensitivity when using this kit, which is illustrated by hit-rate studies (see table). Further advantages of the system include high process reliability and walkaway automation, providing a highly efficient system for purification of viral nucleic acids. The QIAamp Virus BioRobot MDx provides reliable results in downstream applications. High precision using the QIAamp Virus BioRobot MDx Kit PCR product (n=96) Instrument 1 Instrument 2 Mean C T Standard deviation %CV Mean C T Standard deviation %CV Mimic virus 28.26 0.10 0.35 28.38 0.18 0.65 Internal control 31.98 0.16 0.51 31.87 0.22 0.70 Plasma was spiked with armored RNA molecules to mimic RNA viruses (25,000 copies per prep) and processed using the QIAamp Virus BioRobot MDx protocol in replicates of 96 on two separate instruments. An internal control RNA was added to the lysis buffer. The eluted RNA was amplified using a TaqMan® RT-PCR system. Mean C T value, standard deviation, and %CV were calculated for both the mimic virus and the internal control. (Data generated by QIAGEN R&D department.)
The QIAamp Virus BioRobot MDx Kit simplifies isolation of viral RNA and DNA from cell-free body fluids with a fast automated 96-well–plate procedure. No phenol–chloroform extraction is required. Nucleic acids bind specifically to the QIAamp silica-gel membrane while contaminants pass through. PCR inhibitors, such as divalent cations and proteins, are completely removed in two efficient wash steps, leaving pure nucleic acids to be eluted in either water or a buffer provided with the kit. QIAamp technology yields viral RNA and DNA from cell-free body fluids ready to use in PCR and blotting procedures. The fully automated procedure, including bar code reading and complete process documentation, requires less than 2.5 hours to process 96 samples (including a detailed load check of 20 minutes) with no hands-on time during the run. Advantages of the system include high process reliability and walkaway automation, providing a highly efficient system for purification of viral nucleic acids for laboratories. QIAamp sample preparation technology is fully licensed.
Optimized buffers lyse samples, stabilize nucleic acids, and enhance selective nucleic acid adsorption to the QIAamp membrane. Alcohol is added and lysates loaded onto the QIAamp 96-well plate. Wash buffers are used to remove impurities and pure, ready-to-use nucleic acids are then eluted in water or low-salt buffer (see flowchart "Protocol").
