Description
The Saltonase ELISA Kit (5 x 96) is a high-performance assay designed for the quantitative detection of Saltonase endonuclease, including MBG and GMP-grade formats. Engineered for precision, the assay utilizes highly specific monoclonal antibodies to ensure consistent, reliable results with a detection limit of as low as 31.25 pg/mL. Optimized for bioprocessing applications, Saltonase is an endonuclease that removes nucleic acids under high-salt and variable pH conditions. In addition, the ELISA kit is ideal for monitoring residual Saltonase levels in in-process samples and verifying its removal in downstream processing and final product. Each kit includes five pre-coated 96-well microplates in convenient 8-well strip formats and comes with ready-to-use reagents. To extend shelf life and maintain reagent stability, the kit components are packaged in two separate sets. The kit supports a wide quantification range of 31.25–2000 pg/mL and provides sufficient materials for up to 480 measurements.
Caption Assay characteristics Specification Sensitivity LOQ: 30 pg/mL Broad working range 31.25–2000 pg/mL Specificity Monoclonal Antibodies used Low interference from the sample matrix Accuracy RE +/- 20% Processing time 240 min Flexibility 8-well strip plate format
The Saltonase ELISA Kit (5 x 96) utilizes a quantitative sandwich enzyme immunoassay format to ensure high specificity and sensitivity. During the procedure: Standards or samples are added to the wells, allowing any residual Saltonase to bind to the immobilized capture antibody. After a wash step to remove unbound material, a biotin-labeled anti-Saltonase antibody (Detection A) is introduced, binding to the captured enzyme. Following another wash, Streptavidin-Poly-HRP (Detection B) is added. This binds to the biotin-labeled antibody, forming a stable complex. After a final wash, a substrate solution is added. A color reaction develops in proportion to the amount of Saltonase present in the sample. The reaction is stopped, and the absorbance is measured using a standard microplate reader. This precise and reliable method enables accurate quantification of Saltonase across a broad dynamic range, making it ideal for both process development and quality control applications.
Quality Control In accordance with the ISO-certified Quality Management System of QIAGEN, each lot of Saltonase ELISA Kit (5 x 96) is tested against predetermined specifications to ensure consistent product quality. Usage For optimal performance, users should follow the step-by-step protocol and reagent preparation guidelines outlined in the Saltonase ELISA Kit (5 x 96) Handbook. The assay is designed for ease of use with standard laboratory equipment and includes clear instructions for sample preparation, standard curve generation, and result interpretation. Before beginning, ensure all reagents are brought to room temperature and that all standards and samples are run in duplicates or triplicates for reliable results. Please refer to the handbook for complete instructions, reagent handling and troubleshooting recommendations.
