Description
The Incucyte®Neuroactive Kit contain cryopreserved vials of rat cortical neurons and astrocytes isolated from embryonic stage (E18) Sprague-Dawley rats as well as sufficient Incucyte®Neuroburst Orange Lentivirus to perform one 96-well experiment.
The Incucyte®Neuroburst Orange Lentivirus is a lentiviral based live-cell neuronal labeling reagent driven by a synapsin promoter, resulting in the long term expression of a genetically encoded orange fluorescent calcium indicator (mRuby-based) in neuronal cells. The Incucyte®Neuroburst Orange Lentivirus ensures highly efficient, yet non-disruptive labeling of primary or iPSC-derived neurons over days and weeks, and enables the kinetic quantification of neuronal activity and functional connectivity in the presence or absence of astrocytes.
The Incucyte®Neuroburst Orange Lentivirus has been validated for use with the Incucyte®S3 Live-Cell Analysis System configured with an Orange/NIR optical module and the Incucyte®SX5 Live-Cell Analysis System (Cat. No. 4816) for measurements of neuronal activity and functional connectivity with the Incucyte®Neuronal Activity Analysis Software Module (Cat. No. 9600-0032). The Neuroburst Orange Lentivirus is NOT compatible with Incucyte instruments configured with a Green/Red Optical Module, e.g. Cat. No. 4647.
