Thermo Fisher, 043055, Dionex™ CarboPac™ PA100 IC Columns

Name: Dionex™ CarboPac™ PA100 IC Columns
Brand: Thermo Fisher
SKU: 043055

Column Type: Analytical Column, Length (Metric): 250 mm, Diameter (Metric): 4 mm, Unit Size: Each 1, Efficiently resolve oligosaccharides released from therapeutic glycoproteins differing in size, charge, branching, and linkage with the Thermo Scientific™ Dionex™ CarboPac™ PA100 Analytical & Guard Columns. It provides the flexibility to separate oligosaccharides into broad classes depending upon the degree of sialylation, while maintaining the resolution needed for routine analysis based on link

Catalog Number: 043055

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Category

Ion Chromatography Columns

TagsIon Chromatography Columns Chromatography Columns and Cartridges Carbohydrate and Amino-Acid IC Columns

CollectionsThermo Fisher Chromatography Columns & Cartridges (HPLC/UHPLC, GC, IC, SPE)Thermo Fisher Chromatography Columns & Guard Cartridges (HPLC, UHPLC, GC)Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs

Description

Column Type: Analytical Column
Length (Metric): 250 mm
Diameter (Metric): 4 mm
Unit Size: Each 1
The resin of Dionex CarboPac PA100 Columns consists of 5.5μm diameter nonporous beads covered with a fine layer of functionalized Thermo Scientific™ Dionex™ MicroBead™ latex. This pellicular resin structure permits excellent mass transfer, resulting in high-resolution chromatography and rapid re-equilibration. The 3 x 250mm format provides fast separations, and the 0.5mL/minute recommended flow rate results in significant savings in eluent consumption. Predictable, High-Resolution Separations
• Separates closely related oligosaccharides, even isomers, at picomole levels
• Separates neutral and charged oligosaccharides in the same run
• Requires no sample derivatization for detection Use the Thermo Scientific™ Dionex™ CMD™ Carbohydrate Membrane Desalter on line prior to fraction collection to provide easy, automated removal of eluent salts. About the Dionex CarboPac Family of Columns The Dionex CarboPac family offers a selection of high-performance liquid chromatography (HPLC) columns, each optimized for a different class of compounds. Combined with PAD, which provides a means to detect carbohydrates without derivatization, these columns support simple, reliable techniques to separate sugar alcohols, mono- and disaccharides, and poly- and oligosaccharides. Dionex CarboPac columns provide high-resolution separations of:
• Glycoprotein oligosaccharides and complex carbohydrates from dietary fiber, including fructans, maltodextrins, and amylopectins
• Monosaccharides, such as those obtained from glycoprotein hydrolysates or plant hydrolysates
• Mono- and disaccharides

Specifications

General

Column Capacity	90 μeq
Column Construction	PEEK
Flow Rate	1.0 mL/min.
For Use With (Application)	Carbohydrate Analysis
For Use With (Equipment)	HPAE-PAD
Green Features	FSC
Max. Pressure	4000 psi (275 bar)
Particle Size	8.5 μm
Quantity	Each 1
Resin Composition	8.5 μm Diameter Ethylvinylbenzene/Divinylbenzene Substrate (55% Cross-Linking) Agglomerated with 275 nm MicroBead Quaternary Amine Functionalized Latex (6% Cross-Linked).
Solvent Compatibility	100% Compatible with Common Organic Solvents; Ionic Form Eluents: Sodium Acetate and Sodium Hydroxide
Stationary Phase	CarboPac PA100
Temperature (Metric)	4°C to 60°C
Column Type	Analytical Column
Diameter (Metric)	4 mm
Format	Standard Bore
Length (Metric)	250 mm
pH	0 to 14
Unit Size	Each 1

FAQs

Question: Is this an analytical or guard column?

Answer:It is the 4 mm × 250 mm analytical CarboPac PA100 column.

Question: What separation and detection approach does it support?

Answer:It supports strong anion-exchange carbohydrate separation with pulsed amperometric detection.

Question: What are the flow and pressure specifications?

Answer:The recommended flow is 1.0 mL/min and maximum pressure is 4000 psi (275 bar).

Application Note

Dionex™ CarboPac™ PA100 IC Columns is designed for high-performance anion-exchange chromatography of released oligosaccharides and other carbohydrates using pulsed amperometric detection. The Dionex CarboPac PA100 analytical column measures 4 mm × 250 mm and is designed to distinguish oligosaccharides that vary in size, charge, branching and linkage. Its strong anion-exchange packing consists of an ethylvinylbenzene/divinylbenzene polymer substrate agglomerated with quaternary-amine-functionalized MicroBead latex. The manufacturer's resin specification gives an 8.5 µm particle size and a 90 µeq column capacity. Under alkaline sodium hydroxide and sodium acetate elution conditions, the phase can separate neutral and sialylated glycans while allowing closely related oligosaccharide isomers to be resolved. Pulsed amperometric detection enables sensitive carbohydrate analysis without fluorescent derivatization of the sample. The analytical column uses PEEK hardware and has a recommended flow of 1.0 mL/min. Maximum pressure is 4000 psi (275 bar), supported operating pH is 0–14, and the temperature range is 4–60 °C. This 4 mm × 250 mm analytical configuration is distinct from the 4 mm × 50 mm guard version and from narrower-bore members of the same family. It is appropriate for glycoprotein glycan profiling and related chromatography of complex carbohydrate mixtures.

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