Thermo Fisher, 10370088, MEM, NEAA, no glutamine, 10 x 500 mL
Quantity: 10 x 500 mL, Unit Size: Each
Catalog Number: 10370088
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Pack Size10 x 500 mL
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| Cell Line | HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts |
|---|---|
| Cell Type | Primary Rat Astrocytes |
| Concentration | 1 X |
| Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
| Product Line | Gibco |
| Product Type | MEM (Minimum Essential Medium) |
| Quantity | 10 x 500 mL |
| Shelf Life | 24 Months From Date of Manufacture |
| Shipping Condition | Room Temperature |
| Classification | Animal Origin-free |
| Serum Level | Standard Serum Supplementation |
| Sterility | Sterile-filtered |
| Sterilization Method | Sterile-filtered |
| With Additives | Low Glucose |
| Without Additives | No Glutamine |
| Unit Size | Each |
Answer:No. L-glutamine is omitted and must be addressed separately when the culture protocol requires it.
Answer:Yes. The MEM formulation includes non-essential amino acids.
Answer:Ten bottles, each containing 500 mL of liquid medium.
Gibco Minimum Essential Medium with Non-Essential Amino Acids is a liquid basal medium for maintaining appropriate mammalian cell cultures under controlled conditions. This formulation includes non-essential amino acids but omits L-glutamine, allowing a separate glutamine source or alternative supplement to be selected for the specific cell line and experimental design. The package contains ten 500 mL bottles, which supports routine laboratory use while limiting repeated handling of one large container. Standard MEM components include inorganic salts, glucose, vitamins and a pH-indicating system for typical buffered cell-culture procedures. The basal medium is not a complete universal growth formulation: serum, growth supplements or other additives may be required depending on the culture. Work aseptically, use validated storage and warming procedures, and select supplement concentrations based on established cell-specific requirements. Do not substitute this medium for formulations containing added L-glutamine when reproducing protocols that depend on the original glutamine concentration.
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