Thermo Fisher, AM9610, Potassium Acetate (3 M), pH 5.5, RNase-free

Invitrogen Ambion RNase-Free Potassium Acetate is a ready-to-use 3 M solution adjusted to pH 5.5 for nucleic acid preparation and precipitation protocols. The 100 mL bottle eliminates routine preparation of a concentrated acetate stock and supports reproducible control of ionic composition. Potassium acetate can be added to DNA or RNA preparations before ethanol precipitation, with the final salt concentration and solvent ratio set by the validated method. It is also useful in buffers where potassium ions are preferable to added sodium ions. Maintain nuclease-free technique while transferring the solution, especially when processing small amounts of RNA. Potassium acetate should not be substituted blindly into precipitation mixtures containing SDS, because potassium dodecyl sulfate can become insoluble. Select the precipitation conditions based on sample composition rather than treating all salt solutions as interchangeable. The stock is a reagent component rather than a complete extraction or cleanup kit. Store at room temperature with the container securely closed.