Thermo Fisher, AM9820, SDS, 20% Solution, RNase-free

Invitrogen Ambion RNase-Free SDS Solution is a concentrated 20% aqueous sodium dodecyl sulfate reagent for molecular biology workflows. The ready-made solution is supplied in a 250 mL bottle and provides a convenient source of anionic detergent when preparing hybridization and washing buffers. SDS helps disrupt noncovalent interactions and limits unwanted association of proteins with nucleic acids during suitably designed protocols. Its use is particularly relevant to Northern and Southern blot processing, where detergent concentration contributes to wash conditions and membrane background. Dilute the stock with nuclease-free water and appropriate buffer components to achieve the working concentration specified by the assay. Mix thoroughly after dilution, and avoid introducing RNases by using clean vessels, dedicated pipettes and suitable handling practices. This reagent is an SDS solution, not a complete hybridization buffer or ready-to-use electrophoresis loading mix. Store the closed bottle at room temperature and follow laboratory safety practices for detergent handling.