Thermo Fisher, EN0531, RNase A, DNase and protease-free (10 mg/mL)

Thermo Scientific RNase A is an endoribonuclease preparation supplied at 10 mg/mL, with 10 mg total enzyme per package. It is tested to be free of DNase and protease activity and is suitable for removing unwanted RNA during selected plasmid or genomic DNA preparation procedures. RNase A primarily cleaves RNA at cytidine and uridine residues, generating smaller RNA fragments. The enzyme is not a DNA polymerase, a broad-spectrum protease or a selective reagent for degrading DNA. Its reaction behavior depends on salt concentration and other solution conditions; use the recommended working concentration and assess compatibility with downstream assays. At low salt levels, RNase A can also act on double-stranded RNA and the RNA strand in RNA–DNA hybrids. For applications requiring predominantly single-stranded RNA cleavage, higher ionic strength can change selectivity. Handle with appropriate precautions to prevent RNase contamination of RNA-sensitive workspaces. Store the preparation at −20 °C and track aliquot handling and lot details during routine use.