Thermo Fisher, AM9760G, NaCl (5 M), RNase-free, 100 mL

Invitrogen Ambion RNase-Free Sodium Chloride Solution provides a 5 M aqueous NaCl stock for controlled adjustment of ionic strength in nucleic acid experiments. It is supplied as 100 mL of molecular biology-grade solution in a single bottle. Sodium chloride is a common component of hybridization, wash, extraction and nucleic acid storage buffers; its final concentration influences electrostatic interactions and nucleic acid behavior. The concentrated liquid format reduces weighing steps and supports consistent preparation of repeated working solutions. Add the calculated volume to a compatible buffer, then adjust the final volume with nuclease-free water and mix until homogeneous. For RNA work, use dedicated pipette tips and clean containers to preserve the RNase-free status of the reagent during dispensing. A high-concentration NaCl stock should not be confused with a physiological saline solution or a finished RNA purification reagent. Inspect working solutions for any precipitate when combined with other concentrated components. Store the original bottle at room temperature.