Thermo Fisher, 11745500, SuperScript™ III Platinum™ One-Step qRT-PCR Kit w/ROX, 500 reactions

Name: SuperScript™ III Platinum™ One-Step qRT-PCR Kit w/ROX, 500 reactions
Brand: Thermo Fisher
SKU: 11745500

No. of Reactions: 500 Reactions, Unit Size: Each, One-Step qRT-PCR Kits with ROX combine the most powerful reverse transcriptase and DNA polymerase technologies to deliver precise and accurate analysis of gene expression in a convenient, high-throughput one-step format. One-Step qRT-PCR kits with ROX include SuperScript™ III Reverse Transcriptase which produces higher yields of full-length cDNA for broader gene representation, Platinum™ Taq DNA Polymerase which activates faster and more complete

Catalog Number: 11745500

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General

For Use With (Equipment)	7000 System, 7300 System, 7700 System, 7900HT System
No. of Reactions	500 Reactions
Polymerase	Platinum Taq DNA Polymerase
Product Line	Platinum, SuperScript
Product Type	One-Step qRT-PCR Kit
Quantity	500 reactions
Sample Type	RNA
Shipping Condition	Dry Ice
Sufficient For	500 Reactions
Detection Method	Primer-probe
For Use With (Application)	Gene Expression
PCR Method	1-step RT-qPCR
Reaction Speed	Standard
Unit Size	Each

FAQs

Question: How many reactions are included?

Answer:This configuration supports 500 reactions.

Question: Which enzymes are used?

Answer:The kit uses SuperScript III Reverse Transcriptase and Platinum Taq DNA Polymerase.

Question: Is ROX included?

Answer:Yes. ROX passive reference dye is premixed for compatible real-time PCR instruments.

Application Note

SuperScript™ III Platinum™ One-Step qRT-PCR Kit with ROX combines reverse transcription and probe-based real-time PCR in a single tube for RNA target detection. This configuration supports 500 reactions. The system uses SuperScript III Reverse Transcriptase for higher-temperature cDNA synthesis and Platinum Taq DNA Polymerase with hot-start technology for improved amplification specificity. ROX passive reference dye is premixed at a concentration suited to compatible Applied Biosystems real-time PCR instruments. The one-step workflow reduces transfers between reverse-transcription and PCR stages and can be used for gene-expression, pathogen-detection, and viral-RNA applications. Primer-probe detection chemistry is supported, and all amplification steps occur in the same reaction vessel to simplify setup and reduce contamination risk.

Ordering

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