Thermo Fisher, 11753500, CellsDirect™ One-Step qRT-PCR Kit, 500 reactions

No. of Reactions: 500 reactions
Unit Size: Each
The CellsDirect One-Step qRT-PCR Kit delivers highly sensitive and specific real-time qPCR results directly from cells, without the need for an RNA purification step.• Efficiency: offers significant time and cost savings for increased throughput• Convenience: enzyme mix includes both SuperScript™ III Reverse Transcriptase and Platinum™ Taq DNA Polymerase• Sensitivity: no separate RNA isolation step increases sensitivity by minimizing sample loss• Broad detection range: detect targets from samples ranging from a single cell to 10,000 cells• Versatility: compatible with a wide variety of cell lines and samples collected by Laser Capture Microdissection (LCM)Streamline your workflowIn traditional real-time quantitative RT-PCR (qRT-PCR), you first isolate RNA from cells in a time-consuming procedure that can lead to loss of material. Using the CellsDirect One-Step qRT-PCR Kit, you lyse the cells and add the complete lysate directly to a one-step qRT-PCR reaction with minimal handling and sample loss.Ideal for your qRT-PCR application CellsDirect technology is validated for gene expression studies involving limited samples. CellsDirect One-Step qRT-PCR Kits are especially valuable for high-throughput applications by eliminating costly, time-consuming RNA purification steps. The CellsDirect One-Step qRT-PCR Kit may also be used to detect genomic DNA targets. CellsDirect One-Step qRT-PCR Kits may be used in conjunction with hydrolysis probes (e.g., TaqMan™ or LUX™ fluorogenic primers).This kit includes a separate tube of ROX™ Reference Dye. An equivalent kit with ROX Reference Dye included in the 2X Reaction Mix is also available.