Thermo Fisher, 12604021, TrypLE™ Express Enzyme (1X), no phenol red, 500 mL

Name: TrypLE™ Express Enzyme (1X), no phenol red, 500 mL
Brand: Thermo Fisher
SKU: 12604021

Quantity: 500 mL, Unit Size: Each

Catalog Number: 12604021

Pricing

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Category

Non-Animal Origin Dissociation Reagents

Pack Size500 mL

TagsCell Culture Media Supplements and Reagents Cell Culture Reagents Antibiotics and Supplements Dissociation Reagents

CollectionsThermo Fisher Cell Culture Media & Supplements | Serum-Free, Chemically Defined Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 500 mL
Unit Size: Each
Cleaves peptide bonds on the C-terminal sides of lysine and arginine, and is a direct replacement for trypsin. Exceptional purity increases specificity and reduces damage to cells that can be caused by other enzymes present in some trypsin extracts. Used for dissociating a wide range of adherent mammalian cells, including CHO, HEK 293, A529, primary human keratinocytes, and embryonic stem cells.
• Gentle on cells
• Room temperature-stable for 24 months
• Easy to use
• Animal origin-free
• Gentle on cells, purity increases specificity due to the action of a single enzyme and reduces damage caused by cleavage from multiple enzymes in trypsin and other extracts
• Can be directly substituted for trypsin in existing protocols
• Dilution alone inactivates TrypLE Express, avoiding the need for trypsin inhibitors, such as FBSEnergy efficient, less hazardous, sustainable packagingCell Culture, Mammalian Cell Culture

Specifications

General

Cell Type	Mammalian Cells
Chelators	EDTA
Concentration	1 X
Culture Type	Adherent Cell Culture
For Use With (Application)	Clinical Research, Translational Research
Osmolality	Check and Record
Product Line	Gibco
Quantity	500 mL
Remarks	Room Temperature Stable
Shelf Life	24 Months
Shipping Condition	Room Temperature
Source	Animal origin-free
Tested For	In vitro Bioassay
Classification	Animal Origin-free
Form	Liquid
Product Type	Cell Culture Dissociation Reagent
Sterility	Sterile-filtered
Without Additives	No Phenol Red
Unit Size	Each

FAQs

Question: What is the main use of TrypLE Express Enzyme?

Answer:It is used to detach adherent mammalian cells for passaging, harvesting, and related cell-culture workflows.

Question: Does this formulation contain animal-derived trypsin or phenol red?

Answer:No. The enzyme is recombinant and animal-origin free, and this formulation contains no phenol red.

Question: How much solution is supplied?

Answer:This configuration contains 500 mL of ready-to-use 1X solution.

Application Note

TrypLE™ Express Enzyme (1X), no phenol red is used to detach adherent mammalian cells during routine passaging and cell-harvest workflows. The recombinant protease is animal-origin free and provides trypsin-like cleavage activity while avoiding the undefined components associated with porcine trypsin preparations. This 500 mL format is supplied at 1X concentration without phenol red and contains EDTA to support cell dissociation. It can be used with common adherent cell types including CHO and HEK 293 cultures and is suitable for workflows that require a defined dissociation reagent. The solution is sterile-filtered, ships at room temperature and is stable at 15–30°C when protected from light. Its ready-to-use liquid format simplifies routine culture handling because no dilution or reconstitution is required before use.

Ordering

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