Description
Quantity: 20 Reactions
Unit Size: Each
The Pierce RNA 3'-End Desthiobiotinylation Kit is optimized for labeling the 3'-end of single-stranded RNA using T4 RNA ligase. Once labeled, the RNA can be used as a probe or target for gel-shift EMSA reactions, Northern blots and protein-RNA interaction experiments. The desthiobiotinylated RNA also can be used to enrich for RNA binding proteins (RBP) using streptavidin affinity resin, because the desthiobiotin tag binds to streptavidin in a manner which allows gentle elution of the ribonucleoprotein complex. The labeling kit is also included as a component of the Thermo Scientific Pierce Magnetic RNA-Protein Pull-Down Kit. Highlights:
• Non-radioactive– a single biotin labels has similar detection sensitivity as radioactivity
• Easy to use– RNA ligase and optimized reaction buffer are included
• Economical– only a fraction of the cost purchasing synthetic biotinylated RNA probes
• End-labeled– results in minimal disturbance of RNA secondary structure
• Flexible– desthiobiotin label may be used for detection or as an affinity handle for streptavidin resin Includes:
• Desthiobiotinylated cytidine bisphosphate, T4 RNA ligase and buffer, unlabeled RNA oligonucleotide for use as a positive control, a biotinylated RNA probe standard, RNase inhibitor, glycogen, and ligation enhancing reagents Recommended for:
• Northern blotting
• EMSA
• Enrichment of RNA binding proteins (RBP)
