Thermo Fisher, 20340, Detoxi-Gel™ Endotoxin Removing Gel, 1000 mL

Name: Detoxi-Gel™ Endotoxin Removing Gel, 1000 mL
Brand: Thermo Fisher
SKU: 20340

Quantity: 1000 mL, Unit Size: Each, Thermo Scientific™ Detoxi-Gel Endotoxin Removing Gel and Columns provide a quick method to remove endotoxins (lipopolysaccharides or LPS) from samples.

Catalog Number: 20340

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Category

Protein Purification Columns

Pack Size1000 mL

TagsProtein Purification Protein Analysis Reagents Miscellaneous Immobilized Affinity Ligands

CollectionsThermo Fisher Protein Analysis Reagents for Western Blot, ELISA & Proteomics Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 1000 mL
Unit Size: Each
Detoxi-Gel Resin is a beaded agarose support onto which polymixin B has been immobilized. This ligand binds to the lipid A portion of lipopolysaccharides (LPS), which are the dominant form of endotoxins that affect biological samples. Even low levels of endotoxins can be toxic to cells or organisms and must be removed before biological samples can be added to cells for various treatment experiments. Detoxi-Gel Resin provides a quick method for removal of endotoxins from samples. It is available in convenient pre-packed columns and two sizes of slurry for use in small-scale or large-scale processes. Highlights:
• Affinity-based– uses immobilized polymixin B to remove endotoxins by binding to their lipid A domains
• High capacity– 1mL of Detoxi-Gel Resin removes at least 9995 endotoxin units from a 5mL sample containing 10,000 EU of lipopolysaccharide (LPS)
• Reusable– no loss in binding capacity even after 10 regenerations; requires activation and regeneration with 1% sodium deoxycholate (Part No. 89904)
• Testing required– protein recovery is dependent upon protein type and concentration; empirical testing is required to validate the method for particular protein samples
• Versatile– remove endotoxin from protein solutions, cell culture media and pharmacological components Requires:Sodium deoxycholate (Part No. 89904) for resin activation and regeneration.

Specifications

General

Column Type	Affinity, Agarose Resin
Format	Bottle
Quantity	1000 mL
Stationary Phase	Polymixin B
Sufficient For	4000 to 6000 EU per mL Gel
Target	Endotoxin Removal
Capacity (Metric)	4000 to 6000 EU Binding per mL Gel
Product Line	Detoxi-Gel
Type	Endotoxin Removing Gel
Unit Size	Each

Ordering

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