Thermo Fisher, 21030, Pierce™ Gentle Ag/Ab Binding and Elution Buffer Kit

Name: Pierce™ Gentle Ag/Ab Binding and Elution Buffer Kit
Brand: Thermo Fisher
SKU: 21030

Quantity: 200 mL, Unit Size: Each, Thermo Scientific™ Pierce IgG Binding and Elution Buffers ensure high-yield, nondenaturing antibody purification with IgG affinity purification supports, such as Protein A and Protein G agarose beads.

Catalog Number: 21030

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Category

Protein Transfer Buffers

Pack Size200 mL

TagsProtein Analysis Reagents Antibody Affinity Purification Buffers

CollectionsThermo Fisher Protein Analysis Reagents for Western Blot, ELISA & Proteomics Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 200 mL
Unit Size: Each
These products include IgG Binding Buffers specific for each immunoglobulin-binding protein (Protein A, Protein G, Protein A/G). The Pierce IgG Elution Buffer provides efficient dissociation and elution of antibodies from all three IgG-binding proteins, including Protein L. This low-pH elution buffer is also effective for most antibody-antigen or protein-protein affinity purification systems.The Gentle Ag/Ab Binding and Elution Buffer system uses near-neutral, high-salt conditions for elution, enabling even sensitive and labile antibodies (or other proteins) to be eluted from an affinity system without denaturation and inactivation. The phosphate-free binding buffer in this system is a necessary substitute for the Protein A binding buffer (pH 8), which precipitates with the Gentle Ag/Ab Elution Buffer.Finally, the Mouse IgG1 Mild Elution Buffer, when used in combination with Protein A resin, enables selective elution and separation of the IgG1 subclass from total bound mouse IgG. Highlights:
• Three optimized binding buffers– for Protein A, Protein G or Protein A/G
• Two elution buffer options– acidic (pH 2.8) or high salt (near-neutral)
• Special system for mouse IgG1– selective, two-stage elution from Protein A agarose Item-Specific Features:
• Protein A IgG Binding Buffer:pH 8.0; phosphate-based; contains glycerol and EDTA
• Protein A/G IgG Binding Buffer:pH 8.0; phosphate-based; contains EDTA
• Protein G IgG Binding Buffer:pH 5.0; contains 0.02% sodium azide
• IgG Elution Buffer:pH 2.8; amine-based
• Gentle Ag/Ab Binding Buffer:pH 8.0; phosphate-free; contains 0.05% sodium azide
• Gentle Ag/Ab Elution Buffer:pH 6.6; high salt (ionic strength)
• Mouse IgG1 Mild Elution Buffer:pH 6.0; contains 0.02% sodium azide Includes:Kits contain both binding and elution buffers

Specifications

General

Chemical Name or Material	Binding and Elution Buffer
Description	Pierce Gentle Ag/Ab Binding and Elution Buffer Kit
Includes	Sufficient For: Binding and near-neutral elution of antibodies with Protein A or G<br />• Pierce Gentle Ag/Ab Binding Buffer, 100 mL<br />• Pierce Gentle Ag/Ab Elution Buffer, 100 mL<br /><br />Store at 4°C
Physical Form	Liquid
Product Line	Pierce
Quantity	200 mL
pH	8 (Binding Buffer), 6.6 (Elution Buffer)
Unit Size	Each

Ordering

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