Thermo Fisher, 21331020, DMEM/F-12, no glutamine, 500 mL
Quantity: 500 mL, Unit Size: Each
Catalog Number: 21331020
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Category
Pack Size500 mL
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| Cell Type | Mammalian Cells |
|---|---|
| Concentration | 1 X |
| Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
| Product Line | Gibco |
| Product Type | Dulbecco's Modified Eagle Medium (DMEM) |
| Quantity | 500 mL |
| Shelf Life | 12 Months |
| Shipping Condition | Room Temperature |
| Culture Type | Mammalian Cell Culture |
| Form | Liquid |
| Serum Level | Standard Serum Supplementation |
| Sterility | Sterile-filtered |
| With Additives | Phenol Red |
| Without Additives | No HEPES |
| Unit Size | Each |
Answer:No. L-glutamine is not included and can be added separately if required.
Answer:No. This formulation does not contain HEPES.
Answer:The bottle contains 500 mL of sterile-filtered 1X medium.
DMEM/F-12 is a 1:1 mixture of Dulbecco's Modified Eagle Medium and Ham's F-12 Nutrient Mixture for mammalian cell culture. This 500 mL sterile-filtered liquid formulation contains high glucose, sodium pyruvate, sodium bicarbonate, and phenol red but does not contain L-glutamine or HEPES. The combined formulation provides a broader nutrient profile than either base medium alone and is used for many established cell lines as well as primary and specialized culture systems. Because the medium does not contain serum, proteins, lipids, or growth factors, appropriate supplements should be selected for the chosen cell type and experimental design. Sodium bicarbonate serves as the primary buffer, so incubated cultures typically require a controlled CO₂ atmosphere. Store the medium at 2–8 °C protected from light and add glutamine separately when required.
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