| Description | EZ-Link Sulfo-NHS-LC-Biotinylation Kit |
|---|---|
| Labeling Scale | 1 to 10 mg |
| Product Line | EZ-Link |
| Product Type | Sulfo-NHS-LC-Biotinylation Kit |
| Quantity | 10 reactions |
| Labeling Target | Proteins |
| Label or Dye | Biotin |
| Solubility | Water |
| Unit Size | Each |
Answer:The sulfo-NHS ester reacts with primary amines on proteins.
Answer:It supports approximately ten reactions with 1–10 mg of protein per reaction.
Answer:The included Zeba™ Spin Desalting Columns remove excess unreacted reagent.
EZ-Link™ Sulfo-NHS-LC-Biotinylation Kit is designed for covalently labeling antibodies and other proteins with biotin through accessible primary amine groups. The water-soluble sulfo-NHS ester reacts with protein amines to form stable amide bonds, adding a biotin tag separated from the protein by a 22.4 Å spacer arm. The kit provides sufficient materials for approximately ten labeling reactions using 1–10 mg of antibody or other protein per reaction. Its components include 25 mg of EZ-Link Sulfo-NHS-LC-Biotin, a phosphate-buffered-saline preparation pack, ten 5 mL Zeba™ Spin Desalting Columns with a 7,000 molecular-weight cutoff, HABA reagent, and affinity-purified avidin. The desalting columns remove excess unreacted biotinylation reagent after the coupling step, while the HABA/avidin reagents support assessment of biotin incorporation. This integrated workflow is useful for producing biotinylated capture or detection proteins for streptavidin-based purification, immobilization, and binding assays. The reagent is supplied as a kit, not as pre-biotinylated protein. Store biotin reagent and avidin at −20 °C and the remaining components at 4 °C after receipt.
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:
Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.
