Thermo Fisher, 25229, HisPur™ Cobalt Superflow Agarose, 50 mL

Quantity: 50 mL
Unit Size: Each
HisPur Cobalt Superflow Agarose is a highly crosslinked, durable resin that does not compress at flow rates used for medium- to large-scale FPLC purifications. The resin holds up well to a variety of chemicals and pH values, and is compatible with common clean-in-place procedures. Compared to nickel and other ligands commonly used for immobilized metal affinity chromatography (IMAC), cobalt provides greater specificity for His-tagged protein purification. The specific binding of HisPur Cobalt Superflow Agarose results in purer elution fractions, reducing the need for extra polishing steps. Highlights:
• Robust– highly crosslinked 6% agarose Superflow beaded support tolerates linear flow rates up to 1200cm/hour with minimal leaching of cobalt due to a tetradentate chelator
• High yields– binds 20mg of 6xHis-GFP per mL of resin at a linear flow rate of 150cm/hour, can bind greater than 30mg per mL of resin depending on flow rate
• High purity– specificity of cobalt binding to histidine-tag generally results in elution fractions with less than 10% contamination of non-specific proteins
• Compatible– maintains function after exposure to a wide variety of chemicals and pH values
• Cost effective– resin is stable through multiple cycles of cleaning and reuse Recommended for:Large scale FPLC purification of polyhistidine tagged proteins