Thermo Fisher, 28005, Pierce™ Biotin Quantitation Kit

Name: Pierce™ Biotin Quantitation Kit
Brand: Thermo Fisher
SKU: 28005

Quantity: 12 Tests, Unit Size: Each, Thermo Scientific™ Pierce Biotin Quantitation Kit includes aliquots of HABA reagent and biotinylated protein standard for convenient colorimetric determination of biotinylation levels in labeled antibodies and other proteins.

Catalog Number: 28005

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Category

BCA Protein Assay Kits

Pack Size12 tests

TagsProtein Analysis Reagents Crosslinking Labeling and Protein Modification Protein and Antibody Labeling Kits

CollectionsThermo Fisher Protein Analysis Reagents for Western Blot, ELISA & Proteomics Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 12 Tests
Unit Size: Each
Determine the molar ratio of biotin incorporated into a protein using the HABA-avidin method. The HABA dye (4'-hydroxyazobenzene-2-carboxylic acid) binds to avidin to produce a yellow-orange colored complex that absorbs at 500nm. Free biotin present in solution with this avidin-HABA complex will displace the HABA dye and cause the absorbance to decrease.The amount of biotin present can be calculated directly from the decreased absorbance at 500nm. The Thermo Scientific EZ Biotin Quantitation Kit contains pre-measured doses of the Avidin-HABA mixture and biotinylated horseradish peroxidase positive control to simplify reagent preparation and minimize the amount of waste generated. The convenient assay can be performed either in a cuvette or in a microplate.Highlights:
• HABA-avidin complex can be used over a wide range of pH and salt concentrations
• Amount of biotin can be calculated directly from the decreased absorbance at 500nm complexing with the HABA dyeEliminate the need for cumbersome assay equations with a convenient HABA calculator found at www.piercenet.com/HABA/habacalc.cfmIncludes:HABA/Avidin Premix, 24 tubes; Biotinylated HRP, 12 tubes

Specifications

General

Product Line	Pierce
Product Type	Biotin Quantitation Kit
Quantity	12 Tests
Labeling Target	Proteins
Label or Dye	Biotin
Unit Size	Each

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