Thermo Fisher, 34000, Pierce™ CN/DAB Substrate Kit

Quantity: 275 mL
Unit Size: Each
This kit combines two popular chromogenic peroxidase substrate compounds (4-CN and DAB) in a single, stable, 10X stock solution. This CN/DAB solution produces an intense dark black precipitate at sites of bound HRP-conjugated antibodies on probed blots and tissue samples. The Pierce CN/DAB Substrate Kit can be used in Western immunoblotting techniques as well as in dot blotting assays. The kit includes the 10X solution of CN/DAB and accompanying 1X Stable Peroxide Substrate Buffer. Highlights:
• HRP substrate– for detection of horseradish peroxidase activity on solid media via the combined action of CN (4-chloro-1-naphthol) and DAB (3,3'-diaminobenzidine tetrahydrochloride)
• Chromogenic– no special equipment needed for visualization; yields intense black bands that are easy to photograph
• Easy-to-use kit– substrate stock solution and stable peroxide substrate buffer mean that there is no dry powders to measure and dissolve
• Stable– store refrigerated at 4°C for at least one year Includes:10X solution of CN/DAB and the Pierce Stable Peroxide Substrate Buffer